AKR1B10 Monoklonaler Antikörper

AKR1B10 Monoklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

5A4G5

Kat-Nr. : 68327-1-Ig

Synonyme

AKR1B10, AKR1B11, AKR1B12, Aldose reductase like, ALDRLn, ARL 1, ARL1, ARP, hARP, HIS, HSI, SI reductase, Small intestine reductase

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Galerie der Validierungsdaten

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  WB analysis using 68327-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68327-1-Ig (AKR1B10 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat stomach using 68327-1-Ig

  rat stomach tissue were subjected to SDS PAGE followed by western blot with 68327-1-Ig (AKR1B10 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig small intestine using 68327-1-Ig

  pig small intestine tissue were subjected to SDS PAGE followed by western blot with 68327-1-Ig (AKR1B10 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IF Staining of A549 using 68327-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using AKR1B10 antibody (68327-1-Ig, Clone: 5A4G5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, A549-Zellen, Hausschwein-Dünndarmgewebe, Haussschwein-Magengewebe, Ratten-Magengewebe
• Erfolgreiche Detektion in IF: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68327-1-Ig bindet in WB, IF, ELISA AKR1B10 und zeigt Reaktivität mit Hausschwein, human, Ratten

• Getestete Reaktivität: Hausschwein, human, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: AKR1B10 fusion protein Ag24406
• Vollständiger Name: aldo-keto reductase family 1, member B10 (aldose reductase)
• Berechnetes Molekulargewicht: 316 aa, 36 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC008837
• Gene symbol: AKR1B10
• Gene ID (NCBI): 57016
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Aldo-keto reductase family 1 member B10 (AKR1B10), which belongs to the AKR superfamily, consists of 316 amino acids and its gene is located on chromosome 7q33. AKR1B10 was initially identified in liver cancer. It is highly expressed in the small intestine, colon, and adrenal gland.