AKR1C3 Polyklonaler Antikörper

AKR1C3 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11194-1-PBS

Synonyme

17-beta-HSD 5, 17-beta-hydroxysteroid dehydrogenase type 5, 3-alpha-HSD type 2, 3-alpha-HSD type II, brain, 3-alpha-hydroxysteroid dehydrogenase type 2

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  WB analysis using 11194-1-AP (same clone as 11194-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11194-1-AP (AKR1C3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11194-1-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 11194-1-AP (same clone as 11194-1-PBS)

  IP result of anti-AKR1C3 (IP:11194-1-AP, 4ug; Detection:11194-1-AP 1:1000) with HepG2 cells lysate 2400ug. This data was developed using the same antibody clone with 11194-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 11194-1-AP (same clone as 11194-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11194-1-AP (AKR1C3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11194-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 11194-1-AP (same clone as 11194-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11194-1-AP (AKR1C3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11194-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11194-1-PBS bindet in WB, IHC, IP, Indirect ELISA AKR1C3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AKR1C3 fusion protein Ag1674
• Vollständiger Name: aldo-keto reductase family 1, member C3 (3-alpha hydroxysteroid dehydrogenase, type II)
• Berechnetes Molekulargewicht: 323 aa, 37 kDa
• Beobachtetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: BC019230
• Gene symbol: AKR1C3
• Gene ID (NCBI): 8644
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AKR1C3(Aldo-keto reductase family 1 member C3) is also named as DDH1, HSD17B5, KIAA0119, PGFS and belongs to AKR1C family. .In humans, at least four AKR1C isoforms exist: AKR1C1, AKR1C2, AKR1C3, AKR1C4 and AKR1C3 shares >86% sequence identity with these three highly related human AKRs(PMID:18574251). It catalyzes the conversion of aldehydes and ketones to alcohols and androgen, estrogen, PG, xenobiotics metabolism. The rat kidney possesses a dimeric form of 75 kDa(PMID:18574251).