AKR1C3 Rekombinanter Antikörper

AKR1C3 Rekombinant Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242222A11

Kat-Nr. : 84872-5-PBS

Synonyme

17-beta-HSD 5, 17-beta-hydroxysteroid dehydrogenase type 5, 242222A11, 3-alpha-HSD type 2, 3-alpha-HSD type II, brain

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  WB analysis using 84872-5-RR (same clone as 84872-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84872-5-RR (AKR1C3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84872-5-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 84872-5-RR (same clone as 84872-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 84872-5-RR (AKR1C3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84872-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84872-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84872-5-RR against Human AKR1C3 were performed. The affinity constant is 35.8 nM.

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Geprüfte Anwendungen

Produktinformation

84872-5-PBS bindet in WB, IHC, Indirect ELISA AKR1C3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: AKR1C3 fusion protein Ag1674
• Vollständiger Name: aldo-keto reductase family 1, member C3 (3-alpha hydroxysteroid dehydrogenase, type II)
• Berechnetes Molekulargewicht: 323 aa, 37 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC019230
• Gene symbol: AKR1C3
• Gene ID (NCBI): 8644
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AKR1C3(Aldo-keto reductase family 1 member C3) is also named as DDH1, HSD17B5, KIAA0119, PGFS and belongs to AKR1C family. .In humans, at least four AKR1C isoforms exist: AKR1C1, AKR1C2, AKR1C3, AKR1C4 and AKR1C3 shares >86% sequence identity with these three highly related human AKRs(PMID:18574251). It catalyzes the conversion of aldehydes and ketones to alcohols and androgen, estrogen, PG, xenobiotics metabolism.