AKR7A2 Polyklonaler Antikörper

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• KD/KO Validated

AKR7A2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 10730-1-AP

Synonyme

AFAR, AFAR1, AFB1 aldehyde reductase 1, AFB1 AR 1, AFB1 AR1, AKR7, AKR7A2, Aldoketoreductase 7, SSA reductase

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  WB analysis of HEK-293 using 10730-1-AP

  WB result of AKR7A2 antibody (10730-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AKR7A2 transfected HEK-293 cells.

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  WB analysis of mouse liver using 10730-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10730-1-AP (AKR7A2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10730-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10730-1-AP (AKR7A2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 10730-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10730-1-AP (AKR7A2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 10730-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10730-1-AP (AKR7A2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 10730-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10730-1-AP (AKR7A2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, A431-Zellen, HEK-293-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

10730-1-AP bindet in WB, IHC, ELISA AKR7A2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AKR7A2 fusion protein Ag1049
• Vollständiger Name: aldo-keto reductase family 7, member A2 (aflatoxin aldehyde reductase)
• Berechnetes Molekulargewicht: 40 kDa
• Beobachtetes Molekulargewicht: 35-40 kDa
• GenBank-Zugangsnummer: BC007352
• Gene symbol: AKR7A2
• Gene ID (NCBI): 8574
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AKR7A2(Aflatoxin B1 aldehyde reductase member 2) is also named as AFAR, AFAR1, AKR7 and belongs to the aldo/keto reductase family. AKR7A2 enzyme is catalytically active toward aldehydes arising from lipid peroxidation, suggesting a potential role against the consequences of oxidative stress, and representing an important detoxication route in mammalian cells (PMID:22001351).

Publikationen

Species	Application	Title
mouse	WB	Environ Toxicol; Naringin alleviates acetaminophen-induced acute liver injury by activating Nrf2 via CHAC2 upregulation.; Authors - Xiaohan Zhai
human	WB,IHC	Pancreatology; Proteomic analysis of pancreatic ductal adenocarcinoma compared with normal adjacent pancreatic tissue and pancreatic benign cystadenoma.; Authors - Cui Yazhou Y