AKR7A2 Monoklonaler Antikörper

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• KD/KO Validated

AKR7A2 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2E1E8

Kat-Nr. : 66677-1-PBS

Synonyme

AFAR, AFAR1, AFB1 aldehyde reductase 1, AFB1 AR 1, AFB1 AR1, AKR7, AKR7A2, Aldoketoreductase 7, SSA reductase

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  WB analysis of Jurkat using 66677-1-Ig (same clone as 66677-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66677-1-Ig (AKR7A2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66677-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66677-1-Ig (same clone as 66677-1-PBS)

  WB result of AKR7A2 antibody (66677-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AKR7A2 transfected HEK-293 cells. This data was developed using the same antibody clone with 66677-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66677-1-Ig (same clone as 66677-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66677-1-Ig (AKR7A2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66677-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66677-1-Ig (same clone as 66677-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66677-1-Ig (AKR7A2 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66677-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66677-1-Ig (same clone as 66677-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66677-1-Ig (AKR7A2 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66677-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66677-1-Ig (same clone as 66677-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using AKR7A2 antibody (66677-1-Ig, Clone: 2E1E8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66677-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66677-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA AKR7A2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: AKR7A2 fusion protein Ag26105
• Vollständiger Name: aldo-keto reductase family 7, member A2 (aflatoxin aldehyde reductase)
• Berechnetes Molekulargewicht: 40 kDa
• Beobachtetes Molekulargewicht: 35-40 kDa
• GenBank-Zugangsnummer: BC007352
• Gene symbol: AKR7A2
• Gene ID (NCBI): 8574
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AKR7A2(Aflatoxin B1 aldehyde reductase member 2) is also named as AFAR, AFAR1, AKR7 and belongs to the aldo/keto reductase family. AKR7A2 enzyme is catalytically active toward aldehydes arising from lipid peroxidation, suggesting a potential role against the consequences of oxidative stress, and representing an important detoxication route in mammalian cells(PMID:22001351).