AKR7A3 Polyklonaler Antikörper

AKR7A3 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 13209-1-AP

Synonyme

EC:1.-.-.-, AFB1-AR 2, AFB1 AR 2, AFB1 aldehyde reductase 2, AFAR2

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  WB analysis of human liver using 13209-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13209-1-AP (AKR7A3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 13209-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13209-1-AP (AKR7A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 13209-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 13209-1-AP (AKR7A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 13209-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13209-1-AP (AKR7A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Lebergewebe, HepG2-Zellen, humanes Hirngewebe, NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

13209-1-AP bindet in WB, ELISA AKR7A3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AKR7A3 fusion protein Ag3991
• Vollständiger Name: aldo-keto reductase family 7, member A3 (aflatoxin aldehyde reductase)
• Berechnetes Molekulargewicht: 331 aa, 37 kDa
• Beobachtetes Molekulargewicht: 37 kDa and 55-60 kDa
• GenBank-Zugangsnummer: BC025709
• Gene symbol: AKR7A3
• Gene ID (NCBI): 22977
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AKR7A3 belongs to the aldo‐keto reductase (AKR) superfamily, whose primary role is to reduce aldehydes and ketones to generate primary and secondary alcohols, respectively. These enzymes have been shown to play crucial roles in drug metabolism, carcinogen metabolism, and cellular metabolism. Growing evidence suggests that AKR7A3 can play an essential role in the occurrence of cancers, including breast and liver cancers. (PMID: 36951402)

Publikationen

Species	Application	Title
rat	WB	Toxicol Sci; Generation of A New Model Rat: Nrf2 Knockout Rats Are Sensitive to Aflatoxin B1 Toxicity.; Authors - Keiko Taguchi