AKT Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

AKT Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (6)

Anwendung

WB, IP, IHC, IF, FC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

1060

Rezensionen (2)

Kat-Nr. : 10176-2-AP

Synonyme

AKT, AKT1, AKT2, AKT3, PKB, PKB ALPHA, PRKBA, Protein kinase B, Proto oncogene c Akt, RAC, RAC ALPHA, RAC PK alpha

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Galerie der Validierungsdaten

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  WB analysis of MCF-7 using 10176-2-AP

  WB result of AKT antibody (10176-2-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AKT transfected MCF-7 cells.

• 

  WB analysis using 10176-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10176-2-AP (AKT antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa cells using 10176-2-AP

  siRNA AKT1 result from Dr. Eva Martinez-Balibrea. Green:tubulin, Red:10176-2-AP, AKT1.

• 

  WB analysis using 10176-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10176-2-AP (AKT antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10176-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10176-2-AP (AKT antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10176-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10176-2-AP (AKT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 10176-2-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10176-2-AP (AKT1 antibody) at dilution of 1:500.

• 

  IP experiment of HeLa using 10176-2-AP

  IP Result of anti-AKT1 (IP:10176-2-AP, 3ug; Detection:10176-2-AP 1:500) with HeLa cells lysate 2500ug.

• 

  IHC staining of human ovary tumor using 10176-2-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10176-2-AP (AKT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 10176-2-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10176-2-AP (AKT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10176-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10176-2-AP (AKT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10176-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10176-2-AP (AKT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 10176-2-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using AKT antibody (10176-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 10176-2-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 10176-2-AP (AKT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 10176-2-AP

  1X10^6 HeLa cells were stained with 0.2ug AKT1 antibody (10176-2-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, C6-Zellen, HeLa-Zellen, HepG2-Zellen, Maushirngewebe, Mauslebergewebe, MCF-7-Zellen, NIH/3T3-Zellen, Rattenhirngewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen, Maushirngewebe
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 7 publications below
• WB: See 1034 publications below
• IHC: See 31 publications below
• IF: See 16 publications below
• IP: See 6 publications below
• ELISA: See 1 publications below
• CoIP: See 2 publications below

Produktinformation

10176-2-AP bindet in WB, IP, IHC, IF, FC, CoIP, ELISA AKT und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Fisch, hamster, Hausschwein, Huhn, Maus, Ratte, Zebrafisch, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AKT fusion protein Ag0213
• Vollständiger Name: v-akt murine thymoma viral oncogene homolog 1
• Berechnetes Molekulargewicht: 56 kDa
• Beobachtetes Molekulargewicht: 56-62 kDa
• GenBank-Zugangsnummer: BC000479
• Gene symbol: AKT1
• Gene ID (NCBI): 207
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.

Publikationen

Species	Application	Title
human	WB	Cell Res; RAB31 marks and controls an ESCRT-independent exosome pathway.; Authors - Denghui Wei
mouse	WB	Nat Cell Biol; Heat stress activates YAP/TAZ to induce the heat shock transcriptome.; Authors - Min Luo
human	WB	Nat Commun; Genome-wide enhancer-gene regulatory maps link causal variants to target genes underlying human cancer risk; Authors - Pingting Ying
human	WB	Sci Adv; Platelet P-selectin initiates cross-presentation and dendritic cell differentiation in blood monocytes.; Authors - Patrick Han
mouse	WB	Acta Pharm Sin B; Oral nano-formulation improves pancreatic islets dysfunction via lymphatic transport for antidiabetic treatment; Authors - Lin Hou
human	WB	Brain; MMP13 inhibition rescues cognitive decline in Alzheimer transgenic mice via BACE1 regulation.; Authors - Bing-Lin Zhu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hyopil (Verified Customer) (08-23-2021)

Worked well with western blotting

• Applications: Western Blot
• Cell Tissue Type: HAP1

FH

Azita (Verified Customer) (06-02-2021)

Western blot analysis using AKT polyclonal antibody in NSC34 cell line at dilution of 1:500.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: NSC34

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