AKT1/3 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

AKT1/3 Monoklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

50

CloneNo.

3G5E11

Kat-Nr. : 60203-1-Ig

Synonyme

AKT, AKT 1, AKT1, AKT1/3, PKB, PKB ALPHA, PRKBA, Protein kinase B, Proto oncogene c Akt, RAC, RAC ALPHA, RAC PK alpha

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 60203-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60203-1-Ig (AKT1/3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 60203-1-Ig

  WB result of AKT1/3 antibody (60203-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AKT1/3 transfected MCF-7 cells.

• 

  WB analysis of rat liver using 60203-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 60203-1-Ig (AKT1/3 antibody at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 60203-1-Ig

  IP Result of anti-AKT1/3 (IP:60203-1-Ig, 5ug; Detection:60203-1-Ig 1:600) with mouse brain tissue lysate 3440ug.

• 

  IHC staining of human cervical cancer using 60203-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 60203-1-Ig (AKT1/3 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 60203-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60203-1-Ig (AKT1/3 antibody at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 60203-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60203-1-Ig (AKT1/3 antibody at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 60203-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60203-1-Ig (AKT1/3 antibody at dilution of 1:500 (under 10x lens).

• 

  IHC staining of human breast cancer using 60203-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60203-1-Ig (AKT1/3 antibody at dilution of 1:500 (under 40x lens).

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  IF Staining of MCF-7 using 60203-1-Ig

  Immunofluorescent analysis of MCF-7 cells, using AKT1 antibody 60203-1-Ig at 1:50 dilution and Rhodamine-labeled goat anti-mouse IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  FC experiment of MCF-7 using 60203-1-Ig

  1X10^6 MCF-7 cells were stained with 0.2ug AKT1/3 antibody (60203-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1500.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, MCF-7-Zellen, Rattenlebergewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MCF-7-Zellen
• Erfolgreiche Detektion in FC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF): IF : 1:20-1:200
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 49 publications below
• IF: See 1 publications below

Produktinformation

60203-1-Ig bindet in WB, IP, IHC, IF, FC, ELISA AKT1/3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: AKT1/3 fusion protein Ag16695
• Vollständiger Name: v-akt murine thymoma viral oncogene homolog 1
• Berechnetes Molekulargewicht: 56 kDa
• Beobachtetes Molekulargewicht: 56-62 kDa
• GenBank-Zugangsnummer: BC000479
• Gene symbol: AKT1
• Gene ID (NCBI): 207
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The serine-threonine protein kinase AKT is catalytically inactive in serum-starved primary and immortalized fibroblasts. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT, which then phosphorylates and inactivates components of the apoptotic machinery.

Publikationen

Species	Application	Title
mouse	WB	Cell Metab; Exposure to Static Magnetic and Electric Fields Treats Type 2 Diabetes.; Authors - Calvin S Carter
human	WB	Sci Total Environ; Effects of triclosan exposure on stem cells from human exfoliated deciduous teeth (SHED) fate; Authors - Shiwen Deng
mouse	WB	Autophagy; BNIP3L/Nix-induced mitochondrial fission, mitophagy, and impaired myocyte glucose uptake are abrogated by PRKA/PKA phosphorylation.; Authors - Simone C da Silva Rosa
human	WB	Cancer Lett; Endogenous production of C-C motif chemokine ligand 2 by nasopharyngeal carcinoma cells drives radioresistance-associated metastasis.; Authors - Shan-Shan Guo
huamn,mouse	WB	Cell Death Dis; Elaiophylin reduces body weight and lowers glucose levels in obese mice by activating AMPK; Authors - Ruoxuan Bao
mouse	WB	Int J Mol Sci; The Effects of Flavonoid Apigenin on Male Reproductive Health: Inhibition of Spermatogonial Proliferation through Downregulation of Prmt7/Akt3 Pathway.; Authors - Bingyuan Wang