AKT1-Specific Rekombinanter Antikörper

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• KD/KO Validated

AKT1-Specific Rekombinant Antikörper für WB, IHC, FC (Intra), IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, FC (Intra), IP, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

5O9

Kat-Nr. : 80816-1-PBS

Synonyme

AKT, AKT 1, AKT1, 5O9, EC:2.7.11.1

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  WB analysis using 80816-1-RR (same clone as 80816-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80816-1-RR (AKT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80816-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 80816-1-RR (same clone as 80816-1-PBS)

  WB result of AKT1 antibody (80816-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AKT1 transfected MCF-7 cells. This data was developed using the same antibody clone with 80816-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 80816-1-RR (same clone as 80816-1-PBS)

  IP result of anti-AKT1 (IP:80816-1-RR, 4ug; Detection:80816-1-RR 1:2000) with HEK-293 cells lysate 1280 ug. This data was developed using the same antibody clone with 80816-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 80816-1-RR (same clone as 80816-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 80816-1-RR (AKT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80816-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 80816-1-RR (same clone as 80816-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 80816-1-RR (AKT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80816-1-PBS in a different storage buffer formulation.

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  FC experiment of Jurkat using 80816-1-RR (same clone as 80816-1-PBS)

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human AKT1 (80816-1-RR, Clone:5O9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug control antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 80816-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

80816-1-PBS bindet in WB, IHC, FC (Intra), IP, Indirect ELISA AKT1-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: AKT1-Specific fusion protein Ag0213
• Vollständiger Name: v-akt murine thymoma viral oncogene homolog 1
• Berechnetes Molekulargewicht: 56 kDa
• Beobachtetes Molekulargewicht: 56-62 kDa
• GenBank-Zugangsnummer: BC000479
• Gene symbol: AKT1
• Gene ID (NCBI): 207
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. 80816-1-RR recognizes AKT1 specifically.