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AKT3 Rekombinanter Antikörper

AKT3 Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250046E12

Kat-Nr. : 85769-4-PBS

Synonyme

EC:2.7.11.1, PKB gamma, PKBG, PRKBG, Protein kinase Akt 3

Filter:
  • Western Blot (WB) analysis of various lysates using AKT3 Recombinant antibody (85769-4-RR)

    WB analysis using 85769-4-RR (same clone as 85769-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85769-4-RR (AKT3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85769-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85769-4-RR

    Affinity and Kinetic Characterization of 85769-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85769-4-RR against Human AKT3 were performed. The affinity constant is 0.696 nM.

Geprüfte Anwendungen

Produktinformation

85769-4-PBS bindet in WB, Indirect ELISA AKT3 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen AKT3 fusion protein Ag16298
Vollständiger Name v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma)
Berechnetes Molekulargewicht 465 aa, 54 kDa
Beobachtetes Molekulargewicht 60 kDa
GenBank-ZugangsnummerBC121154
Gene symbol AKT3
Gene ID (NCBI) 10000
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AKT3, also named as PKBG, is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. AKT3 is a key modulator of several tumors like melanoma, glioma and ovarian cancer. Active AKT3 increases progressively during melanoma tumor progression with highest levels present in advanced-stage metastatic melanomas.