AKT3 Rekombinanter Antikörper

AKT3 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

250046E12

Kat-Nr. : 85769-4-RR

Synonyme

EC:2.7.11.1, PKB gamma, PKBG, PRKBG, Protein kinase Akt 3

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  WB analysis using 85769-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85769-4-RR (AKT3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 85769-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85769-4-RR against Human AKT3 were performed. The affinity constant is 0.696 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SH-SY5Y-Zellen, Maushirngewebe, Rattenhirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85769-4-RR bindet in WB, ELISA AKT3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: AKT3 fusion protein Ag16298
• Vollständiger Name: v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma)
• Berechnetes Molekulargewicht: 465 aa, 54 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC121154
• Gene symbol: AKT3
• Gene ID (NCBI): 10000
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AKT3, also named as PKBG, is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. AKT3 is a key modulator of several tumors like melanoma, glioma and ovarian cancer. Active AKT3 increases progressively during melanoma tumor progression with highest levels present in advanced-stage metastatic melanomas.