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ALAS2 Polyklonaler Antikörper

ALAS2 Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30539-1-PBS

Synonyme

ALAS-E, ALASE, ALAS E, ALAS 2, 5-aminolevulinic acid synthase 2

Filter:
  • Western Blot (WB) analysis of various lysates using ALAS2 Polyclonal antibody (30539-1-AP)

    WB analysis using 30539-1-AP (same clone as 30539-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30539-1-AP (ALAS2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30539-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using ALAS2 Polyclonal antibody (30539-1-AP)

    IF Staining of HEK-293 using 30539-1-AP (same clone as 30539-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ALAS2 antibody (30539-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 30539-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

30539-1-PBS bindet in WB, IF/ICC, Indirect ELISA ALAS2 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen ALAS2 fusion protein Ag30308
Vollständiger Name aminolevulinate, delta-, synthase 2
Berechnetes Molekulargewicht 587 aa, 65 kDa
Beobachtetes Molekulargewicht65 kDa
GenBank-ZugangsnummerBC030230
Gene symbol ALAS2
Gene ID (NCBI) 212
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The heme biosynthetic pathway begins from delta aminolevulinic acid synthase (ALAS) catalyzing the condensation of glycine and succinyl-CoA to delta aminolevulinic acid (ALA) in the mitochondria. ALAS is coded by two genes: ALAS1 and ALAS2 . ALAS1 is ubiquitously expressed in all cells, and the negative feedback is regulated by the heme pool; however, ALAS2 is specifically expressed only in erythroid cells.