ALAS2 Monoklonaler Antikörper

ALAS2 Monoklonal Antikörper für WB, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2C1H1

Kat-Nr. : 68332-1-PBS

Synonyme

ALASE, ALAS E, ALAS 2, 5-aminolevulinic acid synthase 2, 5-aminolevulinate synthase, erythroid-specific, mitochondrial

---

• 

  WB analysis using 68332-1-Ig (same clone as 68332-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68332-1-Ig (ALAS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68332-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68332-1-Ig (same clone as 68332-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68332-1-Ig (ALAS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68332-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68332-1-Ig (same clone as 68332-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68332-1-Ig (ALAS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68332-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NK-92 using 68332-1-Ig (same clone as 68332-1-PBS)

  NK-92 cells were subjected to SDS PAGE followed by western blot with 68332-1-Ig (ALAS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68332-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP50624-1

  Cytometric bead array standard curve of MP50624-1, ALAS2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68332-1-PBS. Detection antibody: 68332-2-PBS. Standard:Ag13524. Range: 6.25-100 ng/mL.

• 

  Cytometric bead array standard curve of MP50624-2

  Cytometric bead array standard curve of MP50624-2, ALAS2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68332-1-PBS. Detection antibody: 68332-3-PBS. Standard:Ag13524. Range: 3.125-100 ng/mL.

---

Geprüfte Anwendungen

Produktinformation

68332-1-PBS bindet in WB, Cytometric bead array, Indirect ELISA ALAS2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ALAS2 fusion protein Ag13524
• Vollständiger Name: aminolevulinate, delta-, synthase 2
• Berechnetes Molekulargewicht: 587 aa, 65 kDa
• Beobachtetes Molekulargewicht: 60~65 kDa
• GenBank-Zugangsnummer: BC030230
• Gene symbol: ALAS2
• Gene ID (NCBI): 212
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The heme biosynthetic pathway begins from delta aminolevulinic acid synthase (ALAS) catalyzing the condensation of glycine and succinyl-CoA to delta aminolevulinic acid (ALA) in the mitochondria. ALAS is coded by two genes: ALAS1 and ALAS2 . ALAS1 is ubiquitously expressed in all cells, and the negative feedback is regulated by the heme pool; however, ALAS2 is specifically expressed only in erythroid cells.