• Featured Product
  • KD/KO Validated

Albumin Polyklonaler Antikörper

Albumin Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(187)

Kat-Nr. : 16475-1-AP

Synonyme

ALB, Serum albumin, HSA


Filter:
  • Western Blot (WB) analysis of HepG2 cells using Albumin Polyclonal antibody (16475-1-AP)

    WB analysis of HepG2 using 16475-1-AP

    WB result of Albumin antibody (16475-1-AP; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Albumin transfected HepG2 cells.

  • Western Blot (WB) analysis of human plasma using Albumin Polyclonal antibody (16475-1-AP)

    WB analysis of human plasma using 16475-1-AP

    human blood were subjected to SDS PAGE followed by western blot with 16475-1-AP (ALB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Albumin Polyclonal antibody (16475-1-AP)

    WB analysis using 16475-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16475-1-AP (Albumin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse serum using Albumin Polyclonal antibody (16475-1-AP)

    WB analysis of mouse serum using 16475-1-AP

    mouse serum were subjected to SDS PAGE followed by western blot with 16475-1-AP (Albumin Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of human plasma using Albumin Polyclonal antibody (16475-1-AP)

    IP experiment of human plasma using 16475-1-AP

    IP result of anti-Albumin (IP:16475-1-AP, 4ug; Detection:16475-1-AP 1:10000) with human plasma lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human liver tissue using Albumin Polyclonal antibody (16475-1-AP)

    IHC staining of human liver using 16475-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16475-1-AP (Albumin antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using Albumin Polyclonal antibody (16475-1-AP)

    IHC staining of human liver using 16475-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16475-1-AP (Albumin antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using Albumin Polyclonal antibody (16475-1-AP)

    IHC staining of human colon using 16475-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 16475-1-AP (Albumin antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using Albumin Polyclonal antibody (16475-1-AP)

    IHC staining of mouse liver using 16475-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16475-1-AP (Albumin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using Albumin Polyclonal antibody (16475-1-AP)

    IHC staining of mouse liver using 16475-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16475-1-AP (Albumin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using Albumin Polyclonal antibody (16475-1-AP)

    IF Staining of mouse liver using 16475-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Albumin antibody (16475-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using Albumin Polyclonal antibody (16475-1-AP)

    IF Staining of mouse liver using 16475-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Albumin antibody (16475-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using Albumin Polyclonal antibody (16475-1-AP)

    IF Staining of mouse liver using 16475-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using Albumin antibody (16475-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of L02 cells using Albumin Polyclonal antibody (16475-1-AP)

    IF Staining of L02 using 16475-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed L02 cells using Albumin antibody (16475-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

"Albumin Antibodies" Comparison

View side-by-side comparison of Albumin antibodies from other vendors to find the one that best suits your research needs.

Geprüfte Anwendungen

Erfolgreiche Detektion in WBhumanes Blutgewebe, HepG2-Zellen, Mauslebergewebe, Rattenlebergewebe
Erfolgreiche IPhumanes Plasmagewebe
Erfolgreiche Detektion in IHChumanes Lebergewebe, Mauslebergewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF-PMauslebergewebe
Erfolgreiche Detektion in IF/ICCL02-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:50000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:200-1:2000
Immunfluoreszenz (IF)-PIF-P : 1:50-1:500
Immunfluoreszenz (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

16475-1-AP bindet in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA Albumin und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus, Ratte, Ziege
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Albumin fusion protein Ag9569
Vollständiger Name albumin
Berechnetes Molekulargewicht 609 aa, 69 kDa
Beobachtetes Molekulargewicht 66 kDa
GenBank-ZugangsnummerBC034023
Gene symbol Albumin
Gene ID (NCBI) 213
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Albumin is the most abundant protein in blood plasma. Alterations of level of serum albumin are linked to variety of diseases. Albumin is expressed exclusively by well-differentiated hepatocytes, thus anti-albumin has been used to mark hepatocytes. In additon, glycated serum albumin is also a potential diabetes biomarker. The N-terminal 24aa of albumin will be cleaved to generate the mature form of serum albumin with MW 66 kDa. (21388516, 23832071)

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for Albumin antibody 16475-1-APProtokoll herunterladen
IHC protocol for Albumin antibody 16475-1-APProtokoll herunterladenl
IF protocol for Albumin antibody 16475-1-APProtokoll herunterladen
IP protocol for Albumin antibody 16475-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
human,mouseWB

Science

Enterically derived high-density lipoprotein restrains liver injury through the portal vein.

Authors - Yong-Hyun Han
mouseIF

Signal Transduct Target Ther

MDIG-mediated H3K9me3 demethylation upregulates Myc by activating OTX2 and facilitates liver regeneration

Authors - Jinpeng Du
human,mouseWB,IF

Signal Transduct Target Ther

The AKAP12-PKA axis regulates lipid homeostasis during alcohol-associated liver disease

Authors - Chandana Thimme Gowda
human, mouseWB,IF

Nat Immunol

Exosomes mediate the cell-to-cell transmission of IFN-α-induced antiviral activity.

Authors - Li Jianhua J
humanIHC

Immunity

The zinc finger protein Miz1 suppresses liver tumorigenesis by restricting hepatocyte-driven macrophage activation and inflammation.

Authors - Wenjie Zhang
mouseWB

Nat Cell Biol

Manganese regulation of COPII condensation controls circulating lipid homeostasis

Authors - Xiao Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Kamal (Verified Customer) (01-26-2024)

For western blot analysis, antibody worked well at dilution of 1:25000 in 1 X TBST. For immunofluorescence, antibody worked well at dilution of 1:200 in 1 X PBS.

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1:300
  • Cell Tissue Type: Mouse bone marrow
FH

Kamal (Verified Customer) (01-26-2024)

Liver tissues were well stained in provided dilution with 1X PBS.

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Mouse liver tissue