ALCAM Polyklonaler Antikörper

ALCAM Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 21972-1-AP

Synonyme

ALCAM, CD166, CD166 antigen, MEMD

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Galerie der Validierungsdaten

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  WB analysis using 21972-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21972-1-AP (ALCAM antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 21972-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 21972-1-AP (ALCAM antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 21972-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21972-1-AP (ALCAM antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 21972-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 21972-1-AP (ALCAM antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 21972-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 21972-1-AP (ALCAM antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse brain using 21972-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21972-1-AP (ALCAM antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 21972-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21972-1-AP (ALCAM antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 21972-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21972-1-AP (ALCAM Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 21972-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21972-1-AP (ALCAM Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 21972-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 21972-1-AP (ALCAM antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 21972-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 21972-1-AP (ALCAM antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, humanes Lebergewebe, Maushirngewebe, U2OS-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Kolonkarzinomgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

21972-1-AP bindet in WB, IHC, IF, ELISA ALCAM und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ALCAM fusion protein Ag16892
• Vollständiger Name: activated leukocyte cell adhesion molecule
• Berechnetes Molekulargewicht: 583 aa, 65 kDa
• Beobachtetes Molekulargewicht: 100-110 kDa
• GenBank-Zugangsnummer: BC137097
• Gene symbol: ALCAM
• Gene ID (NCBI): 214
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Activated leukocyte cell adhesion molecule (ALCAM, also known as CD166) is a cell adhesion molecule that belongs to the immunoglobulin superfamily. It is involved in cell-cell adhesion through homophilic and heterophilic (to CD6) interactions. ALCAM is widely expressed in a variety of normal tissues. Altered ALCAM expression has been associated with the differentiation state and progression in some neoplasms including melanoma, prostate, colorectal, and breast cancers (PMID: 20461761; 18172759).

Publikationen

Species	Application	Title
human	WB	Cancer Discov; Dynamic glycoprotein hyposialylation promotes chemotherapy evasion and metastatic seeding of quiescent circulating tumor cell clusters in breast cancer; Authors - Nurmaa K Dashzeveg
human	IHC	EBioMedicine; Organoid modelling identifies that DACH1 functions as a tumour promoter in colorectal cancer by modulating BMP signalling.; Authors - Xiang Hu
human	WB,IF	Onco Targets Ther; Alpha-1 Antitrypsin Induces Epithelial-to-Mesenchymal Transition, Endothelial-to-Mesenchymal Transition, and Drug Resistance in Lung Cancer Cells.; Authors - Dong-Ming Wu
human	WB,IHC	Cell Death Dis; PMID: 31024010; Authors - Dong-Ming Wu