ALCAM Monoklonaler Antikörper

ALCAM Monoklonal Antikörper für WB, IHC, IF/ICC, IF-P, IF-Fro, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IF-P, IF-Fro, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1F7D6

Kat-Nr. : 67768-1-PBS

Synonyme

1F7D6, CD166, MEMD

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  WB analysis using 67768-1-Ig (same clone as 67768-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67768-1-Ig (ALCAM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67768-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67768-1-Ig (same clone as 67768-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67768-1-Ig (ALCAM antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67768-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67768-1-Ig (same clone as 67768-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67768-1-Ig (ALCAM antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67768-1-PBS in a different storage buffer formulation.

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  IF Staining of human breast cancer using 67768-1-Ig (same clone as 67768-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using ALCAM antibody (67768-1-Ig, Clone: 1F7D6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67768-1-PBS in a different storage buffer formulation.

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  IF Staining of human breast cancer using 67768-1-Ig (same clone as 67768-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using ALCAM antibody (67768-1-Ig, Clone: 1F7D6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67768-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse breast cancer using 67768-1-Ig (same clone as 67768-1-PBS)

  Immunofluorescent analysis of un-fixed frozen OCT-embedded mouse breast cancer using ALCAM antibody (67768-1-Ig, Clone: 1F7D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67768-1-PBS in a different storage buffer formulation.

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  IF Staining of hTERT-RPE1 using 67768-1-Ig (same clone as 67768-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using ALCAM antibody (67768-1-Ig, Clone: 1F7D6 ) at dilution of 1:500 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 67768-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67768-1-PBS bindet in WB, IHC, IF/ICC, IF-P, IF-Fro, Indirect ELISA ALCAM und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ALCAM fusion protein Ag16892
• Vollständiger Name: activated leukocyte cell adhesion molecule
• Berechnetes Molekulargewicht: 583 aa, 65 kDa
• Beobachtetes Molekulargewicht: 90-100 kDa
• GenBank-Zugangsnummer: BC137097
• Gene symbol: ALCAM
• Gene ID (NCBI): 214
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Activated leukocyte cell adhesion molecule (ALCAM, also known as CD166) is a cell adhesion molecule that belongs to the immunoglobulin superfamily. It is involved in cell-cell adhesion through homophilic and heterophilic (to CD6) interactions. ALCAM is widely expressed in a variety of normal tissues. Altered ALCAM expression has been associated with the differentiation state and progression in some neoplasms including melanoma, prostate, colorectal, and breast cancers (PMID: 20461761; 18172759).