ALCAM Rekombinanter Antikörper

ALCAM Rekombinant Antikörper für WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

241945A4

Kat-Nr. : 84528-1-PBS

Synonyme

241945A4, Activated leukocyte cell adhesion molecule, BEN, CD166, CD166 antigen

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  WB analysis of mouse lung using 84528-1-RR (same clone as 84528-1-PBS)

  mouse lung tissue was subjected to SDS PAGE followed by western blot with 84528-1-RR (ALCAM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84528-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01379-1

  Sandwich ELISA standard curve of MP01379-1, Mouse ALCAM Recombinant Matched Antibody Pair - PBS only. 84528-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1524. 84528-3-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

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  Sample test of MP01379-1

  Serum of six mice was measured. The ALCAM concentration of detected samples was determined to be 43,380.5 pg/mL with a range of 28,091.3-50,380.4 pg/mL.

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  Sample test of MP01379-1

  The mean ALCAM concentration was determined to be 888,916.9 pg/mL in mouse liver tissue extract based on a 3.7 mg/mL extract load and 518,424.5 pg/mL in mouse brain tissue extract based on a 2.0 mg/mL extract load.

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  IHC staining of mouse liver using 84528-1-RR (same clone as 84528-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84528-1-RR (ALCAM antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84528-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 84528-1-RR (same clone as 84528-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84528-1-RR (ALCAM antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84528-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01379-1

  Cytometric bead array standard curve of MP01379-1, MOUSE ALCAM Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84528-1-PBS. Detection antibody: 84528-3-PBS. Standard: Eg1524. Range: 0.781-100 ng/mL.

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Geprüfte Anwendungen

Produktinformation

84528-1-PBS bindet in WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test ALCAM und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ALCAM fusion protein Eg1524
• Vollständiger Name: activated leukocyte cell adhesion molecule
• Berechnetes Molekulargewicht: 65 kDa
• Beobachtetes Molekulargewicht: 90-100 kDa
• GenBank-Zugangsnummer: NM_009655.3
• Gene symbol: Alcam
• Gene ID (NCBI): 11658
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Activated leukocyte cell adhesion molecule (ALCAM, also known as CD166) is a cell adhesion molecule that belongs to the immunoglobulin superfamily. It is involved in cell-cell adhesion through homophilic and heterophilic (to CD6) interactions. ALCAM is widely expressed in a variety of normal tissues. Altered ALCAM expression has been associated with the differentiation state and progression in some neoplasms including melanoma, prostate, colorectal, and breast cancers (PMID: 20461761; 18172759).