P5CS Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

P5CS Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

15

Rezensionen (1)

Kat-Nr. : 17719-1-AP

Synonyme

ALDH18A1, GK, GPR, GSAS, P5CS, PYCS

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Galerie der Validierungsdaten

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  WB analysis using 17719-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17719-1-AP (P5CS antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17719-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17719-1-AP (P5CS antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 17719-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17719-1-AP (P5CS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 17719-1-AP

  IP result of anti-P5CS(IP:17719-1-AP, 4ug; Detection:17719-1-AP 1:1000) with mouse brain tissue lysate 1600 ug.

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  IHC staining of human breast cancer using 17719-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17719-1-AP (P5CS antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 17719-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17719-1-AP (P5CS antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A431 using 17719-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using P5CS antibody (17719-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, HeLa-Zellen, HepG2-Zellen, Maushirngewebe, MCF-7-Zellen, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 15 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

17719-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA P5CS und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: P5CS fusion protein Ag12100
• Vollständiger Name: aldehyde dehydrogenase 18 family, member A1
• Berechnetes Molekulargewicht: 795 aa, 87 kDa
• Beobachtetes Molekulargewicht: 87 kDa
• GenBank-Zugangsnummer: BC106054
• Gene symbol: ALDH18A1
• Gene ID (NCBI): 5832
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Delta 1-pyrroline-5-carboxylate synthetase (P5CS), also named as GSAS, P5CS, PYCS, catalyzes the ATP and the NAD(P)H-dependent conversion of L-glutamate to glutamic gamma-semialdehyde (GSA) which is the metabolic precursor for proline biosynthesis(PMID:8761662). This protein includes 2 domains: glutamate 5-kinase and gamma-glutamyl phosphate reductase. In the N-terminal section, it belongs to the glutamate 5-kinase family and in the C-terminal section, it belongs to the gamma-glutamyl phosphate reductase family. Defects in ALDH18A1 are the cause of cutis laxa, autosomal recessive, type 3A (ARCL3A)(PMID:18478038). It has 2 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
human	WB	Sci Adv; Loss of attachment promotes proline accumulation and excretion in cancer cells; Authors - Steven E Pilley
human	WB	J Exp Med; Inhibiting both proline biosynthesis and lipogenesis synergistically suppresses tumor growth.; Authors - Miao Liu ; KD Validated
mouse	WB	Autophagy; Autophagy loss impedes cancer-associated fibroblast activation via downregulating proline biosynthesis.; Authors - Jingru Bai
human	WB	Proc Natl Acad Sci U S A; Reprogramming of proline and glutamine metabolism contributes to the proliferative and metabolic responses regulated by oncogenic transcription factor c-MYC.; Authors - Liu Wei W
mouse	WB	Int J Mol Sci; Translation Fidelity and Respiration Deficits in CLPP-Deficient Tissues: Mechanistic Insights from Mitochondrial Complexome Profiling; Authors - Jana Key
human	WB	Sci Rep; Proline biosynthesis augments tumor cell growth and aerobic glycolysis: involvement of pyridine nucleotides.; Authors - Wei Liu ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kristian (Verified Customer) (02-05-2020)

The cells were collected, then lyzed five minutes at 95C in SDS-loading buffer.The lysate was run on a 4-20% denaturing SDS-PAGE, then blotted unto a nitrocellulose membrane. The protein was detected using primary antibody diluted 1:1,000 in blotto (PBS, 5% milk, 0.1% Tween-20), then secondary in a 1:10,000 dilution in blotto.Detected using ECL reagents.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human kidney cancer cells (RCC4)

P5CS Antibody Western Blot, validation (1:1000 dilution) in Human kidney cancer cells (RCC4) (Cat no:17719-1-AP)