ALDH1A1 Monoklonaler Antikörper

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• KD/KO Validated

ALDH1A1 Monoklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1A10A2

Kat-Nr. : 60171-1-PBS

Synonyme

1A10A2, 3-deoxyglucosone dehydrogenase, ALDC, Aldehyde dehydrogenase 1A1, ALDH 1A1

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  WB analysis using 60171-1-Ig (same clone as 60171-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60171-1-Ig (ALDH1A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 60171-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 60171-1-Ig (same clone as 60171-1-PBS)

  WB result of ALDH1A1 antibody (60171-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ALDH1A1 transfected K-562 cells. This data was developed using the same antibody clone with 60171-1-PBS in a different storage buffer formulation.

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  WB analysis of pig liver using 60171-1-Ig (same clone as 60171-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 60171-1-Ig (ALDH1A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60171-1-PBS in a different storage buffer formulation.

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  WB analysis of rat liver using 60171-1-Ig (same clone as 60171-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 60171-1-Ig (ALDH1A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60171-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 60171-1-Ig (same clone as 60171-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60171-1-Ig (ALDH1A1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 60171-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 60171-1-Ig (same clone as 60171-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60171-1-Ig (ALDH1A1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 60171-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 60171-1-Ig (same clone as 60171-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ALDH1A1 antibody (60171-1-Ig, Clone: 1A10A2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60171-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 60171-1-Ig (same clone as 60171-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human ALDH1A1 (60171-1-Ig, Clone:1A10A2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 60171-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

60171-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA ALDH1A1 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ALDH1A1 fusion protein Ag8551
• Vollständiger Name: aldehyde dehydrogenase 1 family, member A1
• Berechnetes Molekulargewicht: 501 aa, 55 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC001505
• Gene symbol: ALDH1A1
• Gene ID (NCBI): 216
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ALDH1A1(Aldehyde dehydrogenase family 1 member A1 ), also named as ALDC, ALDH1 and PUMB1, belongs to the aldehyde dehydrogenase family. The ALDH1A1 gene encodes a liver cytosolic isoform of acetaldehyde dehydrogenase, an enzyme involved in the major pathway of alcohol metabolism after alcohol dehydrogenase. ALDH1A1 plays a critical role in protection against oxidative stress-induced cytotoxicity in lens epithelial cells(PMID:19296407). And it is important for multiple biological activities including drug resistance, cell differentiation, and oxidative stress response(PMID:19025616). As a novel cancer stem cell marker, ALDH1A1 can be used for tumors whose corresponding normal tissues express ALDH1A1 in relatively restricted or limited levels such as breast, lung, ovarian or colon cancer(PMID: 20422001).