ALDH1A1 Monoklonaler Antikörper

ALDH1A1 Monoklonal Antikörper für IF

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

IF

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

1A10A2

Rezensionen (1)

Kat-Nr. : CL594-60171

Synonyme

ALDC, ALDH E1, ALDH1, ALDH11, ALDH1A1, ALHDII, PUMB1, RALDH 1, RALDH1, Retinal dehydrogenase 1

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Galerie der Validierungsdaten

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  IF Staining of HeLa using CL594-60171

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594 ALDH1A1 antibody (CL594-60171, Clone: 1A10A2 ) at dilution of 1:100.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-60171 bindet in IF ALDH1A1 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ALDH1A1 fusion protein Ag8551
• Vollständiger Name: aldehyde dehydrogenase 1 family, member A1
• Berechnetes Molekulargewicht: 501 aa, 55 kDa
• GenBank-Zugangsnummer: BC001505
• Gene symbol: ALDH1A1
• Gene ID (NCBI): 216
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ALDH1A1(Aldehyde dehydrogenase family 1 member A1 ), also named as ALDC, ALDH1 and PUMB1, belongs to the aldehyde dehydrogenase family. The ALDH1A1 gene encodes a liver cytosolic isoform of acetaldehyde dehydrogenase, an enzyme involved in the major pathway of alcohol metabolism after alcohol dehydrogenase. ALDH1A1 plays a critical role in protection against oxidative stress-induced cytotoxicity in lens epithelial cells(PMID:19296407). And it is important for multiple biological activities including drug resistance, cell differentiation, and oxidative stress response(PMID:19025616). As a novel cancer stem cell marker, ALDH1A1 can be used for tumors whose corresponding normal tissues express ALDH1A1 in relatively restricted or limited levels such as breast, lung, ovarian or colon cancer(PMID: 20422001). This antibody is CL594(Ex/Em 593 nm/614 nm) conjugated.

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Vanessa (Verified Customer) (10-12-2023)

Tried many times different concentrations (until 1:50) and no signal.

• Applications: Immunohistochemistry, Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Brain