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ALDH1A2 Rekombinanter Antikörper

ALDH1A2 Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

251371D10

Kat-Nr. : 86518-1-PBS

Synonyme

ALDH1A 2, EC:1.2.1.36, RALDH 2, RALDH(II), RalDH2

Filter:
  • Western Blot (WB) analysis of various lysates using ALDH1A2 Recombinant antibody (86518-1-RR)

    WB analysis using 86518-1-RR (same clone as 86518-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86518-1-RR (ALDH1A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86518-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of K-562 cells using ALDH1A2 Recombinant antibody (86518-1-RR)

    IF Staining of K-562 using 86518-1-RR (same clone as 86518-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using ALDH1A2 antibody (86518-1-RR, Clone: 251371D10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86518-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86518-1-RR

    Affinity and Kinetic Characterization of 86518-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86518-1-RR against Human ALDH1A2 were performed. The affinity constant is 0.179 nM.

Geprüfte Anwendungen

Produktinformation

86518-1-PBS bindet in WB, IF/ICC, Indirect ELISA ALDH1A2 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen ALDH1A2 fusion protein Ag5062
Vollständiger Name aldehyde dehydrogenase 1 family, member A2
Berechnetes Molekulargewicht 518 aa, 57 kDa
Beobachtetes Molekulargewicht 53-57 kDa
GenBank-ZugangsnummerBC030589
Gene symbol ALDH1A2
Gene ID (NCBI) 8854
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ALDH1A2(aldehyde dehydrogenase family 1 member A2) is also named as RALDH2(retinal dehydrogenase 2) and belongs to the aldehyde dehydrogenase family. It is a cytosolic homotetramer(56.7 kDa subunits) exhibiting complex expression patterns throughout embryonic development. ALDH1A2 plays a role in retinoid metabolism during embryonic development where it is considered to be the major retinoic acid-synthesizing enzyme during early embryogenesis(PMID:20735668). Its expression is reduced in primary prostate tumors compared with normal prostate tissue suggested that it is normally expressed in the epithelial compartment of prostate tissue(PMID:16166285).It has 2 isoforms produced by alternative splicing.