ALDH1A2 Rekombinanter Antikörper

ALDH1A2 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

251371D10

Kat-Nr. : 86518-1-RR

Synonyme

ALDH1A 2, EC:1.2.1.36, RALDH 2, RALDH(II), RalDH2

---

• 

  WB analysis using 86518-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86518-1-RR (ALDH1A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of K-562 using 86518-1-RR

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using ALDH1A2 antibody (86518-1-RR, Clone: 251371D10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  Affinity and Kinetic Characterization of 86518-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86518-1-RR against Human ALDH1A2 were performed. The affinity constant is 0.179 nM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausmilzgewebe, Maushodengewebe, Rattenmilzgewebe, Rattenhodengewebe
• Erfolgreiche Detektion in IF/ICC: K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86518-1-RR bindet in WB, IF/ICC, ELISA ALDH1A2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ALDH1A2 fusion protein Ag5062
• Vollständiger Name: aldehyde dehydrogenase 1 family, member A2
• Berechnetes Molekulargewicht: 518 aa, 57 kDa
• Beobachtetes Molekulargewicht: 53-57 kDa
• GenBank-Zugangsnummer: BC030589
• Gene symbol: ALDH1A2
• Gene ID (NCBI): 8854
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ALDH1A2(aldehyde dehydrogenase family 1 member A2) is also named as RALDH2(retinal dehydrogenase 2) and belongs to the aldehyde dehydrogenase family. It is a cytosolic homotetramer(56.7 kDa subunits) exhibiting complex expression patterns throughout embryonic development. ALDH1A2 plays a role in retinoid metabolism during embryonic development where it is considered to be the major retinoic acid-synthesizing enzyme during early embryogenesis(PMID:20735668). Its expression is reduced in primary prostate tumors compared with normal prostate tissue suggested that it is normally expressed in the epithelial compartment of prostate tissue(PMID:16166285).It has 2 isoforms produced by alternative splicing.