ALDH1B1 Rekombinanter Antikörper

ALDH1B1 Rekombinant Antikörper für WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242944E7

Kat-Nr. : 85426-2-PBS

Synonyme

Aldehyde dehydrogenase 5, Aldehyde dehydrogenase X, mitochondrial, ALDH5, ALDHX, EC:1.2.1.3

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  WB analysis using 85426-2-RR (same clone as 85426-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85426-2-RR (ALDH1B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85426-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA sample test of MP01995-1

  The mean ALDH1B1 concentration was determined to be 124.73 ng/mL in HeLa cell extract based on a 1.80 mg/mL extract load, 191.33 ng/mL in HepG2 cell extract based on a 1.80 mg/mL extract load and 74.97 ng/mL in K-562 cell extract based on a 2.50 mg/mL extract load.

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  Sandwich ELISA standard curve of MP01995-1

  Sandwich ELISA standard curve of MP01995-1, Human ALDH1B1 Recombinant Matched Antibody Pair - PBS only. 85426-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag7144. 85426-2-PBS was HRP conjugated as the detection antibody. Range: 0.791-50 ng/mL.

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  IHC staining of mouse testis using 85426-2-RR (same clone as 85426-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 85426-2-RR (ALDH1B1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85426-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 85426-2-RR (same clone as 85426-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 85426-2-RR (ALDH1B1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85426-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 85426-2-RR (same clone as 85426-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85426-2-RR (ALDH1B1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85426-2-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 85426-2-RR (same clone as 85426-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85426-2-RR (ALDH1B1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85426-2-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP01995-1

  Cytometric bead array standard curve of MP01995-1, ALDH1B1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85426-1-PBS. Detection antibody: 85426-2-PBS. Standard: Ag7144. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 85426-2-RR

  Biolayer interferometry (BLl) kinetic assays of 85426-2-RR against Human ALDH1B1 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

85426-2-PBS bindet in WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA ALDH1B1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ALDH1B1 fusion protein Ag7144
• Vollständiger Name: aldehyde dehydrogenase 1 family, member B1
• Berechnetes Molekulargewicht: 57 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: BC001619
• Gene symbol: ALDH1B1
• Gene ID (NCBI): 219
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Aldehyde dehydrogenases (ALDHs) belong to a superfamily of NAD(P)+-dependent enzymes, which catalyze the oxidation of endogenous and exogenous aldehydes to their corresponding acids. ALDH1B1 is a mitochondrial ALDH that it is dramatically upregulated in human colonic adenocarcinoma, making it a potential biomarker for human colon cancer.(PMID:21216231). ALDH1B1 is a homotetramer expressed in various adult and fetal human tissues including liver, testis, kidney, skeletal muscle, heart, placenta, brain and lung(PMID:18611112).