ALDH1L1 Monoklonaler Antikörper

ALDH1L1 Monoklonal Antikörper für WB, IHC, IF-P, IF-Fro, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF-P, IF-Fro, ELISA

Konjugation

Unkonjugiert

CloneNo.

1C1A3

Kat-Nr. : 68018-1-PBS

Synonyme

10 FTHFDH, 10-FTHFDH, 1C1A3, ALDH1L 1, Cytosolic 10-formyltetrahydrofolate dehydrogenase

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  WB analysis of human milk using 68018-1-Ig (same clone as 68018-1-PBS)

  human milk were subjected to SDS PAGE followed by western blot with 68018-1-Ig (ALDH1L1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68018-1-PBS in a different storage buffer formulation.

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  WB analysis of pig liver using 68018-1-Ig (same clone as 68018-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 68018-1-Ig (ALDH1L1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68018-1-PBS in a different storage buffer formulation.

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  WB analysis of rat liver using 68018-1-Ig (same clone as 68018-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 68018-1-Ig (ALDH1L1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68018-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 68018-1-Ig (same clone as 68018-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 68018-1-Ig (ALDH1L1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68018-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 68018-1-Ig (same clone as 68018-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68018-1-Ig (ALDH1L1 antibody) at dilution of 1:32000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68018-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 68018-1-Ig (same clone as 68018-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68018-1-Ig (ALDH1L1 antibody) at dilution of 1:32000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68018-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 68018-1-Ig (same clone as 68018-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using ALDH1L1 antibody (68018-1-Ig, Clone: 1C1A3 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68018-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 68018-1-Ig (same clone as 68018-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using ALDH1L1 antibody (68018-1-Ig, Clone: 1C1A3 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-3). This data was developed using the same antibody clone with 68018-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68018-1-PBS bindet in WB, IHC, IF-P, IF-Fro, ELISA ALDH1L1 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ALDH1L1 fusion protein Ag13190
• Vollständiger Name: aldehyde dehydrogenase 1 family, member L1
• Berechnetes Molekulargewicht: 99 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC027241
• Gene symbol: ALDH1L1
• Gene ID (NCBI): 10840
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Aldehyde dehydrogenase 1 family member L1 (ALDH1L1), belongs to the aldehyde dehydrogenase family of proteins and is responsible for formate oxidation in vivo. Loss of ALDH1L1 is associated with decreased apoptosis, increased cell motility, and cancer progression. ALDH1L1 catalyzes the conversion of 10-formyltetrahydrofolate, nicotinamide adenine dinucleotide phosphate (NADP+), and water to tetrahydrofolate, NADPH, and carbon dioxide. ALDH1L1 has some isoforms with MW of 99, 88 and 55 kDa.