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ALDH2 Rekombinanter Antikörper

ALDH2 Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

251287E9

Kat-Nr. : 86429-5-PBS

Synonyme

ALDH 2, ALDH class 2, ALDH E2, ALDHE2, ALDH-E2

Filter:
  • Western Blot (WB) analysis of various lysates using ALDH2 Recombinant antibody (86429-5-RR)

    WB analysis using 86429-5-RR (same clone as 86429-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86429-5-RR (ALDH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86429-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of C2C12 cells using ALDH2 Recombinant antibody (86429-5-RR)

    IF Staining of C2C12 using 86429-5-RR (same clone as 86429-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using ALDH2 antibody (86429-5-RR, Clone: 251287E9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86429-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using ALDH2 Recombinant antibody (86429-5-RR)

    IF Staining of A549 using 86429-5-RR (same clone as 86429-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using ALDH2 antibody (86429-5-RR, Clone: 251287E9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86429-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86429-5-RR

    Affinity and Kinetic Characterization of 86429-5-RR

    Biolayer interferometry (BLl) kinetic assays of 86429-5-RR against Human ALDH2 were performed. The affinity constant is below 1 pM.

Geprüfte Anwendungen

Produktinformation

86429-5-PBS bindet in WB, IF/ICC, Indirect ELISA ALDH2 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen ALDH2 fusion protein Ag7452
Vollständiger Name aldehyde dehydrogenase 2 family (mitochondrial)
Berechnetes Molekulargewicht 56 kDa
Beobachtetes Molekulargewicht 50-55 kDa
GenBank-ZugangsnummerBC002967
Gene symbol ALDH2
Gene ID (NCBI) 217
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Acetaldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. It mediates NADP+-dependent oxidation of aldehydes into acids during detoxification of alcohol-derived acetaldehyde; lipid peroxidation; and metabolism of corticosteroids, biogenic amines and neurotransmitters. Genetic variation in ALDH2 is responsible for individual differences in responses to drinking alcohol. Thus, the absence of this enzyme is linked to alcohol intolerance and a reduced risk for alcoholism-related liver disease.