ALDH3A1 Polyklonaler Antikörper

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• KD/KO Validated

ALDH3A1 Polyklonal Antikörper für FC, IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

20

Kat-Nr. : 15578-1-AP

Synonyme

Aldehyde dehydrogenase 3, ALDH3, ALDH3A1, ALDHIII

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Galerie der Validierungsdaten

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  WB analysis of A549 using 15578-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 15578-1-AP (ALDH3A1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 15578-1-AP

  WB result of ALDH3A1 antibody (15578-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ALDH3A1 transfected A549 cells.

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  WB analysis of A549 using 15578-1-AP

  WB result of ALDH3A1 antibody (15578-1-AP; 1:6000; room temperature for 1.5 hours) with wild-type and ALDH3A1 knockout A549 cells.

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  IP experiment of A549 using 15578-1-AP

  IP result of anti-ALDH3A1(IP:15578-1-AP, 4ug; Detection:15578-1-AP 1:3000) with A549 cells lysate 1480 ug.

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  IF Staining of HEK-293T using 15578-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293T cells using ALDH3A1 antibody (15578-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HEK-293 using 15578-1-AP

  1X10^6 HEK-293 cells were intracellularly stained with 0.25 ug Anti-Human ALDH3A1 (15578-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.25 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen
• Erfolgreiche IP: A549-Zellen
• Erfolgreiche Detektion in IF: HEK-293T-Zellen
• Erfolgreiche Detektion in FC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:20000-1:100000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 3 publications below
• IF: See 10 publications below
• FC: See 2 publications below

Produktinformation

15578-1-AP bindet in WB, IP, IHC, IF, FC, ELISA ALDH3A1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ALDH3A1 fusion protein Ag7977
• Vollständiger Name: aldehyde dehydrogenase 3 family, memberA1
• Berechnetes Molekulargewicht: 50 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC004370
• Gene symbol: ALDH3A1
• Gene ID (NCBI): 218
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ALDH3A1(Aldehyde dehydrogenase, dimeric NADP-preferring), also named as ALDH3, is a member of the ALDH superfamily, which consists of NAD(P)+-dependent enzymes that catalyse the oxidation of a wide variety of endogenously produced and exogenous aldehydes to their corresponding acids. It is expressed at high concentrations in the mammalian cornea and it is believed that it protects this vital tissue and the rest of the eye against UVlight-induced damage.(PMID:12943535). It can exsit as a dimer(PMID:16919242).

Publikationen

Species	Application	Title
human	WB	Mol Cell; Kynurenine importation by SLC7A11 propagates anti-ferroptotic signaling.; Authors - Alessandra Fiore
rat	IF	Nat Commun; Fiber reinforced GelMA hydrogel to induce the regeneration of corneal stroma.; Authors - Bin Kong
human	IHC	Adv Healthc Mater; Sustained Release of TPCA-1 from Silk Fibroin Hydrogels Preserves Keratocyte Phenotype and Promotes Corneal Regeneration by Inhibiting Interleukin-1β Signaling.; Authors - Wei Zhang
human	WB	Cell Chem Biol; Lipid-derived electrophiles mediate the effects of chemotherapeutic topoisomerase I poisons.; Authors - Amy Flor
human	IF	Antioxid Redox Signal; Elucidation of the AGR2 interactome in esophageal adenocarcinoma cells identifies a redox sensitive chaperone hub for the quality control of MUC-5AC.; Authors - Jack C Worfolk
human	IF,WB	ACS Biomater Sci Eng; Regulation of the Keratocyte Phenotype and Cell Behavior Derived from Human Induced Pluripotent Stem Cells by Substrate Stiffness; Authors - Lan Zhang

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C