ALDH3A1 Rekombinanter Antikörper

ALDH3A1 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

243134D8

Kat-Nr. : 85725-1-RR

Synonyme

AL3A1, Aldehyde dehydrogenase, dimeric NADP-preferring, ALDH3, ALDHIII, EC:1.2.1.5

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  WB analysis using 85725-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85725-1-RR (ALDH3A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of BxPC-3 using 85725-1-RR

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 85725-1-RR (ALDH3A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 85725-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85725-1-RR against Human ALDH3A1 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, BxPC-3-Zellen, Maus-Magengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85725-1-RR bindet in WB, ELISA ALDH3A1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ALDH3A1 fusion protein Ag7977
• Vollständiger Name: aldehyde dehydrogenase 3 family, memberA1
• Berechnetes Molekulargewicht: 50 kDa
• Beobachtetes Molekulargewicht: 48-52 kDa
• GenBank-Zugangsnummer: BC004370
• Gene symbol: ALDH3A1
• Gene ID (NCBI): 218
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ALDH3A1(Aldehyde dehydrogenase, dimeric NADP-preferring), also named as ALDH3, is a member of the ALDH superfamily, which consists of NAD(P)+-dependent enzymes that catalyse the oxidation of a wide variety of endogenously produced and exogenous aldehydes to their corresponding acids. It is expressed at high concentrations in the mammalian cornea and it is believed that it protects this vital tissue and the rest of the eye against UVlight-induced damage.(PMID:12943535). It can exsit as a dimer(PMID:16919242).