ALDH3B2 Polyklonaler Antikörper

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• KD/KO Validated

ALDH3B2 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 15746-1-AP

Synonyme

EC:1.2.1.3, ALDH8, Aldehyde dehydrogenase 8

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  WB analysis of mouse lung using 15746-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 15746-1-AP (ALDH3B2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 15746-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15746-1-AP (ALDH3B2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of BxPC-3 using 15746-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 15746-1-AP (ALDH3B2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human adrenal gland using 15746-1-AP

  Immunohistochemical analysis of paraffin-embedded human adrenal gland using 15746-1-AP (ALDH3B2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human adrenal gland using 15746-1-AP

  Immunohistochemical analysis of paraffin-embedded human adrenal gland using 15746-1-AP (ALDH3B2 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 15746-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ALDH3B2 antibody (15746-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslungengewebe, BxPC-3-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Nebennierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 1 publications below

Produktinformation

15746-1-AP bindet in WB, IHC, IF/ICC, ELISA ALDH3B2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ALDH3B2 fusion protein Ag8197
• Vollständiger Name: aldehyde dehydrogenase 3 family, member B2
• Berechnetes Molekulargewicht: 395 aa, 43 kDa
• Beobachtetes Molekulargewicht: 43 kDa
• GenBank-Zugangsnummer: BC007685
• Gene symbol: ALDH3B2
• Gene ID (NCBI): 222
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ALDH3B2(aldehyde dehydrogenase family 3 member B2) is also named as ALDH8(aldehyde dehydrogenase 8) and belongs to the aldehyde dehydrogenase family. The ALDH3B2 gene contains an in-frame stop codon at the seventeenth codon position from the first methionine,suggesting that ALDH3B2 may be a pseudogene(PMID:15237855) and encodes the human salivary gland and placental ALDH(PMID: 22206977). This protein shares 83% sequence identity with ALDH3B1(PMID:18611112 ).

Publikationen

Species	Application	Title
human	WB,IHC	Cell Death Dis; Aldehyde dehydrogenase 3B2 promotes the proliferation and invasion of cholangiocarcinoma by increasing Integrin Beta 1 expression; Authors - Yue Wang ; KD Validated
human	WB	Authors -
human	WB	Int J Mol Sci; Targeting Retinaldehyde Dehydrogenases to Enhance Temozolomide Therapy in Glioblastoma; Authors - Rafael Jiménez
human	WB	Biochem Pharmacol; β-Elemene inhibits epithelial-mesenchymal transformation in non-small cell lung cancer by targeting ALDH3B2/RPSA axis; Authors - Jiawei Zhou ; KD Validated