ALDH4A1 Rekombinanter Antikörper

ALDH4A1 Rekombinant Antikörper für WB, Cytometric bead array, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, Cytometric bead array, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

250957F8

Kat-Nr. : 86297-1-PBS

Synonyme

ALDH4, ALDH4A 1, Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial, EC:1.2.1.88, L-glutamate gamma-semialdehyde dehydrogenase

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  WB analysis using 86297-1-RR (same clone as 86297-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86297-1-RR (ALDH4A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86297-1-PBS in a different storage buffer formulation.

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  Sample test of MP02370-1

  The mean ALDH4A1 concentration was determined to be 29.8 ng/mL in K-562 cell extract based on a 3.0 mg/mL extract load.

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  Cytometric bead array standard curve of MP02370-1

  Cytometric bead array standard curve of MP02370-1, ALDH4A1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86297-2-PBS. Detection antibody: 86297-1-PBS. Standard: Ag2147. Range: 0.312-40 ng/mL.

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  Affinity and Kinetic Characterization of 86297-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86297-1-RR against Human ALDH4A1 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

86297-1-PBS bindet in WB, Cytometric bead array, Indirect ELISA, Sample test ALDH4A1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ALDH4A1 fusion protein Ag2147
• Vollständiger Name: aldehyde dehydrogenase 4 family, member A1
• Berechnetes Molekulargewicht: 563 aa, 62 kDa
• Beobachtetes Molekulargewicht: 62 kDa
• GenBank-Zugangsnummer: BC023600
• Gene symbol: ALDH4A1
• Gene ID (NCBI): 8659
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ALDH4A1(Aldehyde dehydrogenase family 4 member A1) is also named as ALDH4, P5CDH,P5C dehydrogenase and belongs to the aldehyde dehydrogenase family. P5CDH is a mitochondrial matrix NAD(+)-dependent dehydrogenase which catalyzes the second step of the proline degradation pathway, converting pyrroline-5-carboxylate to glutamate. Defects in ALDH4A1 are the cause of hyperprolinemia type 2 (HP-2).