ALDH5A1 Polyklonaler Antikörper

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• KD/KO Validated

ALDH5A1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 17319-1-AP

Synonyme

ALDH5A1, SSADH, SSDH

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  WB analysis using 17319-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17319-1-AP (ALDH5A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 17319-1-AP

  WB result of ALDH5A1 antibody (17319-1-AP; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ALDH5A1 transfected HepG2 cells.

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  IHC staining of human liver cancer using 17319-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17319-1-AP (ALDH5A1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 17319-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17319-1-AP (ALDH5A1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 17319-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17319-1-AP (ALDH5A1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 17319-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17319-1-AP (ALDH5A1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 17319-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17319-1-AP (ALDH5A1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 17319-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17319-1-AP (ALDH5A1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of A431 using 17319-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using 17319-1-AP (ALDH5A1 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

17319-1-AP bindet in WB, IHC, IF/ICC, ELISA ALDH5A1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ALDH5A1 fusion protein Ag11293
• Vollständiger Name: aldehyde dehydrogenase 5 family, member A1
• Berechnetes Molekulargewicht: 535 aa, 57 kDa
• Beobachtetes Molekulargewicht: 51-59 kDa
• GenBank-Zugangsnummer: BC034321
• Gene symbol: ALDH5A1
• Gene ID (NCBI): 7915
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	J Ethnopharmacol; Multi-omics and chemical profiling approaches to understand the material foundation and pharmacological mechanism of Sophorae Tonkinensis Radix et Rhizome-induced liver injury in mice; Authors - Si-Wei Rao
human	IHC	Cancer Biol Ther; Decreased expression of ALDH5A1 predicts prognosis in patients with ovarian cancer.; Authors - Xun Tian
mouse	WB	Neuropharmacology; A role of GABAA receptor α1 subunit in the hippocampus for rapid-acting antidepressant-like effects of ketamine; Authors - Xiao-Hui Tang
human,mouse	WB,IF	Aging (Albany NY); Mitochondrial-related genes PDK2, CHDH, and ALDH5A1 served as a diagnostic signature and correlated with immune cell infiltration in ulcerative colitis; Authors - Qian Yang
mouse	WB	Ecotoxicol Environ Saf; 1,2-Dichloroethane causes anxiety and cognitive dysfunction in mice by disturbing GABA metabolism and inhibiting the cAMP-PKA-CREB signaling pathway; Authors - Yuchen Qin
human	WB	Sci Rep; Identifying ADME-related gene signature for immune landscape and prognosis in KIRC by single-cell and spatial transcriptome analysis; Authors - Hongyun Wang