ALDH5A1 Monoklonaler Antikörper

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ALDH5A1 Monoklonal Antikörper für WB, IHC, IF-P, FC (Intra), Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF-P, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1B9H10

Kat-Nr. : 67347-1-PBS

Synonyme

Succinate-semialdehyde dehydrogenase, mitochondrial, SSDH, SSADH, NAD(+)-dependent succinic semialdehyde dehydrogenase, EC:1.2.1.24

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  WB analysis of L02 using 67347-1-Ig (same clone as 67347-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 67347-1-Ig (ALDH5A1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67347-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 67347-1-Ig (same clone as 67347-1-PBS)

  WB result of ALDH5A1 antibody (67347-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ALDH5A1 transfected HepG2 cells. This data was developed using the same antibody clone with 67347-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 67347-1-Ig (same clone as 67347-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67347-1-Ig (ALDH5A1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67347-1-PBS in a different storage buffer formulation.

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  WB analysis of pig brain using 67347-1-Ig (same clone as 67347-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67347-1-Ig (ALDH5A1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67347-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67347-1-Ig (same clone as 67347-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67347-1-Ig (ALDH5A1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67347-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67347-1-Ig (same clone as 67347-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67347-1-Ig (ALDH5A1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67347-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67347-1-Ig (same clone as 67347-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67347-1-Ig (ALDH5A1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67347-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67347-1-Ig (same clone as 67347-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67347-1-Ig (ALDH5A1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67347-1-PBS in a different storage buffer formulation.

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  IF Staining of human liver cancer using 67347-1-Ig (same clone as 67347-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using ALDH5A1 antibody (67347-1-Ig, Clone: 1B9H10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). DAPI (blue). This data was developed using the same antibody clone with 67347-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse liver using 67347-1-Ig (same clone as 67347-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using ALDH5A1 antibody (67347-1-Ig, Clone: 1B9H10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67347-1-PBS in a different storage buffer formulation.

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  FC experiment of A431 using 67347-1-Ig (same clone as 67347-1-PBS)

  1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human ALDH5A1 (67347-1-Ig, Clone:1B9H10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67347-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67347-1-PBS bindet in WB, IHC, IF-P, FC (Intra), Indirect ELISA ALDH5A1 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ALDH5A1 fusion protein Ag11155
• Vollständiger Name: aldehyde dehydrogenase 5 family, member A1
• Berechnetes Molekulargewicht: 535 aa, 57 kDa
• Beobachtetes Molekulargewicht: 51 kDa
• GenBank-Zugangsnummer: BC034321
• Gene symbol: ALDH5A1
• Gene ID (NCBI): 7915
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.