ALDH7A1 Rekombinanter Antikörper

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• KD/KO Validated

ALDH7A1 Rekombinant Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

CloneNo.

242363B11

Kat-Nr. : 84871-4-RR

Synonyme

242363B11, ALDH7A 1, Alpha-AASA dehydrogenase, Alpha-aminoadipic semialdehyde dehydrogenase, Antiquitin-1

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  WB analysis using 84871-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84871-4-RR (ALDH7A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 84871-4-RR

  WB result of ALDH7A1 antibody (84871-4-RR; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ALDH7A1 transfected HepG2 cells.

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  IP experiment of HeLa using 84871-4-RR

  IP result of anti-ALDH7A1 (IP:84871-4-RR, 4ug; Detection:84871-4-RR 1:3000) with HeLa cells lysate 1200 ug.

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  IHC staining of human ovary cancer using 84871-4-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84871-4-RR (ALDH7A1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 84871-4-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84871-4-RR (ALDH7A1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 84871-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84871-4-RR against Human ALDH7A1 were performed. The affinity constant is 0.253 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A431-Zellen, A549-Zellen, HEK-293T-Zellen, HepG2-Zellen, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: human ovary cancer tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84871-4-RR bindet in WB, IHC, IP, ELISA ALDH7A1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ALDH7A1 fusion protein Ag0383
• Vollständiger Name: aldehyde dehydrogenase 7 family, member A1
• Berechnetes Molekulargewicht: 55 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC002515
• Gene symbol: ALDH7A1
• Gene ID (NCBI): 501
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ALDH7A1(Alpha-aminoadipic semialdehyde dehydrogenase) is also named as ATQ1 and belongs to the aldehyde dehydrogenase family. It plays a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation.