ALDH9A1 Polyklonaler Antikörper

ALDH9A1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 26621-1-AP

Synonyme

EC:1.2.1.19, ALDH9, ALDH7, ALDH4, 4-trimethylaminobutyraldehyde dehydrogenase, N-terminally processed

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  WB analysis of NIH/3T3 using 26621-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 26621-1-AP (ALDH9A1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 26621-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 26621-1-AP (ALDH9A1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 26621-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26621-1-AP (ALDH9A1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 26621-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26621-1-AP (ALDH9A1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 26621-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26621-1-AP (ALDH9A1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 26621-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26621-1-AP (ALDH9A1 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 26621-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ALDH9A1 antibody (26621-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NIH/3T3-Zellen, HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Produktinformation

26621-1-AP bindet in WB, IHC, IF/ICC, ELISA ALDH9A1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ALDH9A1 fusion protein Ag24312
• Vollständiger Name: aldehyde dehydrogenase 9 family, member A1
• Berechnetes Molekulargewicht: 518 aa, 56 kDa
• Beobachtetes Molekulargewicht: 46 kDa
• GenBank-Zugangsnummer: BC151140
• Gene symbol: ALDH9A1
• Gene ID (NCBI): 223
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Aldehyde dehydrogenase 9 family member A1(ALDH9A1), belongs to the aldehyde dehydrogenase family of proteins. ALDH9A1 has a high activity for oxidation of gamma-aminobutyraldehyde and other amino aldehydes. ALDH9A1 catalyzes the dehydrogenation of gamma-aminobutyraldehyde to gamma-aminobutyric acid. ALDH9A1 has 3 isoforms with the molecular mass of 46-56 kDa.

Publikationen

Species	Application	Title
human	WB	Cancers (Basel); Mitochondrial Protein Cox7b Is a Metabolic Sensor Driving Brain-Specific Metastasis of Human Breast Cancer Cells; Authors - Marine C N M Blackman
human	WB	Proteomes; Utilizing Optimized Tools to Investigate PTM Crosstalk: Identifying Potential PTM Crosstalk of Acetylated Mitochondrial Proteins.; Authors - Henrick Horita
human	WB,IHC	Mol Carcinog; C1QBP Promotes Prostate Cancer Progression and Lipid Accumulation by Negatively Regulating ALDH9A1; Authors - Xinyu Liu ; KD Validated