ALDOA Monoklonaler Antikörper

ALDOA Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

CloneNo.

1D6C2

Kat-Nr. : 67453-1-Ig

Synonyme

Lung cancer antigen NY-LU-1, Fructose-bisphosphate aldolase A, EC:4.1.2.13, Aldolase A, ALDA

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  WB analysis using 67453-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67453-1-Ig (ALDOA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis using 67453-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67453-1-Ig (ALDOA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 67453-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67453-1-Ig (ALDOA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 67453-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67453-1-Ig (ALDOA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 67453-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ALDOA antibody (67453-1-Ig, Clone: 1D6C2 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, 4T1-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, NIH/3T3-Zellen, Hausschwein-Lebergewebe
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

67453-1-Ig bindet in WB, IHC, IF/ICC, ELISA ALDOA und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ALDOA fusion protein Ag17952
• Vollständiger Name: aldolase A, fructose-bisphosphate
• Berechnetes Molekulargewicht: 39 kDa
• Beobachtetes Molekulargewicht: 35-40 kDa
• GenBank-Zugangsnummer: BC016800
• Gene symbol: ALDOA
• Gene ID (NCBI): 226
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ALDOA(Fructose-bisphosphate aldolase A) is also named as lung cancer antigen NY-LU-1,muscle-type aldolase,ALDA and belongs to the class I fructose-bisphosphate aldolase family.The ALDOA gene encodes a homotetrameric protein(PMID:14615364).It is found in skeletal muscle and red blood cells, and functions in glycolysis, promoting the reversible cleavage of FBP to triose phosphate, G-3-P and DHAP. ALDOA is upregulated in various cancers, including human lung squamous and renal cell carcinomas(PMID:19937139).

Publikationen

Species	Application	Title
	WB	Food Chem; Phosphorylation and acetylation responses of glycolytic enzymes in meat to different chilling rates; Authors - Yuqiang Bai
human	WB	Oncogene; HMGB1/SET/HAT1 complex-mediated SASH1 repression drives glycolysis and metastasis in lung adenocarcinoma; Authors - Fan Kou
human	WB,IHC,IF	Cell Rep; A pro-metastatic tRNA fragment drives aldolase A oligomerization to enhance aerobic glycolysis in lung adenocarcinoma; Authors - Qinglin Wang
human	IHC	J Cancer; Establishment of Chemotherapy Prediction Model Based on Hypoxia-Related Genes for Oral Cancer; Authors - Chuhuan Zhou