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ALG1 Polyklonaler Antikörper

ALG1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12872-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of HeLa cells using ALG1 Polyclonal antibody (12872-1-AP)

    WB analysis of HeLa using 12872-1-AP (same clone as 12872-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 12872-1-AP (ALG1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12872-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using ALG1 Polyclonal antibody (12872-1-AP)

    IP experiment of HeLa using 12872-1-AP (same clone as 12872-1-PBS)

    IP result of anti-ALG1 (IP:12872-1-AP, 4ug; Detection:12872-1-AP 1:500) with HeLa cells lysate 3080 ug. This data was developed using the same antibody clone with 12872-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using ALG1 Polyclonal antibody (12872-1-AP)

    IHC staining of human gliomas using 12872-1-AP (same clone as 12872-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12872-1-AP (ALG1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12872-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using ALG1 Polyclonal antibody (12872-1-AP)

    IHC staining of human gliomas using 12872-1-AP (same clone as 12872-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12872-1-AP (ALG1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12872-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ALG1 Polyclonal antibody (12872-1-AP)

    IF Staining of HeLa using 12872-1-AP (same clone as 12872-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 12872-1-AP (ALG1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12872-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

12872-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA ALG1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen ALG1 fusion protein Ag3540
Vollständiger Name asparagine-linked glycosylation 1, beta-1,4-mannosyltransferase homolog (S. cerevisiae)
Berechnetes Molekulargewicht 464 aa, 53 kDa
Beobachtetes Molekulargewicht 47-53 kDa
GenBank-ZugangsnummerBC031095
Gene symbol ALG1
Gene ID (NCBI) 56052
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.