ALG5 Polyklonaler Antikörper

ALG5 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16046-1-PBS

Synonyme

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  WB analysis of human kidney using 16046-1-AP (same clone as 16046-1-PBS)

  human kidney tissue were subjected to SDS PAGE followed by western blot with 16046-1-AP (ALG5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16046-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 16046-1-AP (same clone as 16046-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16046-1-AP (ALG5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16046-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 16046-1-AP (same clone as 16046-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16046-1-AP (ALG5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16046-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 16046-1-AP (same clone as 16046-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16046-1-AP (ALG5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16046-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 16046-1-AP (same clone as 16046-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16046-1-AP (ALG5 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 16046-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 16046-1-AP (same clone as 16046-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16046-1-AP (ALG5 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 16046-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 16046-1-AP (same clone as 16046-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta using 16046-1-AP (ALG5 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16046-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 16046-1-AP (same clone as 16046-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta using 16046-1-AP (ALG5 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16046-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 16046-1-AP (same clone as 16046-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 16046-1-AP (ALG5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16046-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

16046-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA ALG5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ALG5 fusion protein Ag8990
• Vollständiger Name: asparagine-linked glycosylation 5, dolichyl-phosphate beta-glucosyltransferase homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 324 aa, 37 kDa
• Beobachtetes Molekulargewicht: 37 kDa
• GenBank-Zugangsnummer: BC012531
• Gene symbol: ALG5
• Gene ID (NCBI): 29880
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Dolichyl-phosphate beta-glucosyltransferase(ALG5), belongs to the family of glycosyltransferases, specifically the hexosyltransferases. ALG5 is widely expressed in pancreas, placenta, liver, heart, brain, kidney, skeletal muscle, and lung. ALG5 can catalyze the chemical reaction between UDP-glucose and UDP with dolichyl phosphate.