Alix Monoklonaler Antikörper

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• KD/KO Validated

Alix Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(18)

CloneNo.

1H9D9

Kat-Nr. : 67715-1-Ig

Synonyme

PDCD6IP, 1H9D9, AIP1, ALG-2-interacting protein 1, ALG-2-interacting protein X

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  WB analysis using 67715-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67715-1-Ig (Alix antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67715-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67715-1-Ig (Alix antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 67715-1-Ig

  WB result of Alix antibody (67715-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Alix transfected HEK-293 cells.

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  IHC staining of human stomach cancer using 67715-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67715-1-Ig (Alix antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 67715-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67715-1-Ig (Alix antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 67715-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Alix antibody (67715-1-Ig, Clone: 1H9D9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HSC-T6 cells, 4T1-Zellen, A431-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, NIH/3T3-Zellen, PC-12-Zellen
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:20000
• Immunhistochemie (IHC): IHC : 1:4000-1:16000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 16 publications below
• IF: See 1 publications below

Produktinformation

67715-1-Ig bindet in WB, IHC, IF/ICC, ELISA Alix und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Alix fusion protein Ag30437
• Vollständiger Name: programmed cell death 6 interacting protein
• Berechnetes Molekulargewicht: 868 aa, 96 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC020066
• Gene symbol: Alix
• Gene ID (NCBI): 10015
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ALG-2-interacting protein 1 (ALIX), also known as AIP1 or Hp95, is encoded by PDCD6IP gene and is involved in cell death through mechanisms involving its binding partner ALG-2 (apoptosis-linked gene-2). ALG-2 is a 22-kDa protein containing five serially repetitive EF-hand structures and is defined as a regulator of calcium-induced apoptosis following endoplasmic reticulum (ER) stress. ALIX interacts with ALG-2 through its C-terminal proline-rich region and participates in formation of multivesicular bodies. Recent finding suggest that ALIX is a critical component of caspase 9 activation and apoptosis triggered by calcium.

Publikationen

Species	Application	Title
human	WB	Stem Cell Res Ther; Tremella polysaccharide microneedles loaded with magnetic dental pulp stem cell intracellular vesicles used for androgenic alopecia; Authors - Yonghao Liu
human,mouse	WB,IF	Environ Pollut; TDCPP Disrupts ALG-2/ALIX-Mediated ESCRT-III Recruitment: Implications for Lysosomal Membrane Repair and Neurotoxicity; Authors - Tingting Li
human	WB	Cell Mol Biol Lett; Downregulation of exosomal miR-7-5p promotes breast cancer migration and invasion by targeting RYK and participating in the atypical WNT signalling pathway; Authors - Zhaoyi Liang
mouse,human	WB	J Thromb Haemost; Cancer-associated fibroblasts promote venous thrombosis through podoplanin/CLEC-2 interaction in podoplanin-negative lung cancer mouse model; Authors - Toshiaki Shirai
human	WB	Psychiatry Clin Neurosci; Human in vivo evidence of reduced astrocyte activation and neuroinflammation in patients with treatment-resistant depression following electroconvulsive therapy; Authors - Shu-Xian Xu
human	WB	J Microsc; A method for extraction of exosomes from breast tumour cells and characterisation by transmission electron microscopy; Authors - Zhiang Liu