ALKBH2 Polyklonaler Antikörper

ALKBH2 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 17809-1-AP

Synonyme

ABH2, ALKBH2, Oxy DC1

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Galerie der Validierungsdaten

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  WB analysis using 17809-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17809-1-AP (ALKBH2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Caco-2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

17809-1-AP bindet in WB, ELISA ALKBH2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ALKBH2 fusion protein Ag12108
• Vollständiger Name: alkB, alkylation repair homolog 2 (E. coli)
• Berechnetes Molekulargewicht: 261 aa, 29 kDa
• Beobachtetes Molekulargewicht: 30-35 kDa
• GenBank-Zugangsnummer: BC070489
• Gene symbol: ALKBH2
• Gene ID (NCBI): 121642
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Escherichia coli AlkB is a DNA repair enzyme that represents the founding member of the AlkB family of non-heme iron-dependent dioxygenases. The human genome encodes nine AlkB homologs termed AlkB homolog 1 through 8 (ALKBH1 through 8) plus a ninth member termed fat mass and obesity associated (FTO). Bacterial AlkB as well as human ALKBH2 and ALKBH3 catalyze the direct reversal of DNA methylation damage through a distinct oxidative demethylation reaction. Importantly, ALKBH2 has been shown to play a role in DNA alkylation damage repair in human, mouse and plant cells. Full-length ALKBH2 has a molecular weight of about 30kDa and ALKBH2 variant encoded by an alternatively spliced transcript with a frameshift deletion of the C-terminal dioxygenase domain has a molecular weight of about 25kDa (PMID:22079122).