ALKBH3 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ALKBH3 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 12292-1-AP

Synonyme

ABH3, ALKBH3, DEPC 1, DEPC1, PCA1, Prostate cancer antigen 1

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  WB analysis using 12292-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12292-1-AP (ALKBH3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 12292-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12292-1-AP (ALKBH3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 12292-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12292-1-AP (ALKBH3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 12292-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12292-1-AP (ALKBH3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 12292-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 12292-1-AP (ALKBH3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human prostate cancer using 12292-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12292-1-AP (ALKBH3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 12292-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12292-1-AP (ALKBH3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 12292-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12292-1-AP (ALKBH3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat stomach using 12292-1-AP

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 12292-1-AP (ALKBH3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse stomach using 12292-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 12292-1-AP (ALKBH3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 12292-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12292-1-AP (ALKBH3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 12292-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12292-1-AP (ALKBH3 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, Mausherzgewebe, Mausnierengewebe, Rattenherzgewebe
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Pankreaskarzinomgewebe, humanes Prostatakarzinomgewebe, humanes Hodengewebe, Maus-Magengewebe, Ratten-Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 3 publications below

Produktinformation

12292-1-AP bindet in WB, IHC, ELISA ALKBH3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ALKBH3 fusion protein Ag2982
• Vollständiger Name: alkB, alkylation repair homolog 3 (E. coli)
• Berechnetes Molekulargewicht: 286 aa, 33 kDa
• Beobachtetes Molekulargewicht: 22-25 kDa, 33kDa
• GenBank-Zugangsnummer: BC015155
• Gene symbol: ALKBH3
• Gene ID (NCBI): 221120
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ALKBH3, also named as ABH3 and DEPC-1, is a dioxygenase that mediates demethylation of DNA and RNA containing 1-methyladenosine (m1A). It has a strong preference for single-stranded. ALKBH3 is requires molecular oxygen, alpha-ketoglutarate and iron. This gene has some isoforms with MW 19-25 kDa and 33 kDa.

Publikationen

Species	Application	Title
human	WB,IHC	J Gastroenterol Hepatol; ALKBH3 expression is associated with tumor recurrence and unfavorable prognosis in hepatocellular carcinoma.; Authors - Qian Wang ; KD Validated
human	WB	Cell Biosci; m1A demethylase Alkbh3 regulates neurogenesis through m1A demethylation of Mmp15 mRNA; Authors - Huan Wang
human	WB	Virol J; Evaluation of ALKBH2 and ALKBH3 gene regulation in patients with adult T-cell leukemia/lymphoma; Authors - Yuji Wada
human	IHC	Biomedicines; The Roles of AGTRAP, ALKBH3, DIVERSIN, NEDD8 and RRM1 in Glioblastoma Pathophysiology and Prognosis; Authors - Claudia Alexandra Dumitru
human	IHC	Clin Transl Med; PUS7-dependent pseudouridylation of ALKBH3 mRNA inhibits gastric cancer progression; Authors - Yongxia Chang