ALKBH4 Polyklonaler Antikörper

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• KD/KO Validated

ALKBH4 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 19882-1-AP

Synonyme

ABH4, Alpha-ketoglutarate-dependent dioxygenase alkB homolog 4, DNA N6-methyl adenine demethylase ALKBH4, EC:1.14.11.-, EC:1.14.11.51

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  WB analysis of mouse kidney using 19882-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 19882-1-AP (ALKBH4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 19882-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 19882-1-AP (ALKBH4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 19882-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 19882-1-AP (ALKBH4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 19882-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 19882-1-AP (ALKBH4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 19882-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ALKBH4 antibody (19882-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, K-562-Zellen, MCF-7-Zellen, Maus-Eierstockgewebe
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 5 publications below
• IF: See 1 publications below

Produktinformation

19882-1-AP bindet in WB, IF/ICC, ELISA ALKBH4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ALKBH4 fusion protein Ag8041
• Vollständiger Name: alkB, alkylation repair homolog 4 (E. coli)
• Berechnetes Molekulargewicht: 34 kDa
• Beobachtetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: BC002820
• Gene symbol: ALKBH4
• Gene ID (NCBI): 54784
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Cancer Discov; Identification of BBOX1 as a Therapeutic Target in Triple-Negative Breast Cancer.; Authors - Chengheng Liao ; KD Validated
human	WB,IF	Sci Rep; ALKBH overexpression in head and neck cancer: potential target for novel anticancer therapy.; Authors - Tomaš Pilžys ; KD Validated
human	WB	Acta Biochim Pol; Selected ALKBH dioxygenases are overexpressed in salivary gland tumours; Authors - Michał Marcinkowski
mouse,human	WB	Water (Basel); ALKBH4 Stabilization Is Required for Arsenic-Induced 6mA DNA Methylation Inhibition, Keratinocyte Malignant Transformation, and Tumorigenicity; Authors - Yan-Hong Cui ; KD Validated
pig	WB	Sci Rep; The proteomic profile is altered but not repaired after bariatric surgery in type 2 diabetes pigs; Authors - Karolina Ferenc