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ALKBH5 Rekombinanter Antikörper

ALKBH5 Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2K9

Kat-Nr. : 82083-1-PBS

Synonyme

RNA demethylase ALKBH5, EC:1.14.11.53, Alpha-ketoglutarate-dependent dioxygenase alkB homolog 5, ALKBH 5, 2K9

Filter:
  • Western Blot (WB) analysis of HeLa cells using ALKBH5 Recombinant antibody (82083-1-RR)

    WB analysis of HeLa using 82083-1-RR (same clone as 82083-1-PBS)

    WB result of ALKBH5 antibody (82083-1-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ALKBH5 transfected HeLa cells. This data was developed using the same antibody clone with 82083-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ALKBH5 Recombinant antibody (82083-1-RR)

    WB analysis using 82083-1-RR (same clone as 82083-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82083-1-RR (ALKBH5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82083-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using ALKBH5 Recombinant antibody (82083-1-RR)

    IF Staining of U-251 using 82083-1-RR (same clone as 82083-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using ALKBH5 antibody (82083-1-RR, Clone: 2K9 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82083-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82083-1-RR

    Affinity and Kinetic Characterization of 82083-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82083-1-RR against Human ALKBH5 were performed. The affinity constant is below 1 pM.

Geprüfte Anwendungen

Produktinformation

82083-1-PBS bindet in WB, IF/ICC, Indirect ELISA ALKBH5 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen ALKBH5 fusion protein Ag10516
Vollständiger Name alkB, alkylation repair homolog 5 (E. coli)
Berechnetes Molekulargewicht 458 aa, 51 kDa
Beobachtetes Molekulargewicht 40-50 kDa
GenBank-ZugangsnummerBC062339
Gene symbol ALKBH5
Gene ID (NCBI) 54890
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ALKBH5, also named as ABH5 and OFOXD1, is a Dioxygenase that demethylates RNA by oxidative demethylation. It is a mammalian demethylase that oxidatively reverses m6A in mRNA in vitro and in vivo. ALKBH5 is required for spermatogenesis. There're some isoforms of ALKBH5 with MW 40-44 kDa and 52 kDa.