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ALKBH8 Polyklonaler Antikörper

ALKBH8 Polyklonal Antikörper für IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 25566-1-PBS

Synonyme

ABH8, EC:2.1.1.229, Probable alpha-ketoglutarate-dependent dioxygenase ABH8, S-adenosyl-L-methionine-dependent tRNA methyltransferase ABH8, tRNA (carboxymethyluridine(34)-5-O)-methyltransferase ABH8

Filter:
  • Immunohistochemistry (IHC) staining of rat kidney tissue using ALKBH8 Polyclonal antibody (25566-1-AP)

    IHC staining of rat kidney using 25566-1-AP (same clone as 25566-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 25566-1-AP (ALKBH8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25566-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using ALKBH8 Polyclonal antibody (25566-1-AP)

    IF Staining of HEK-293 using 25566-1-AP (same clone as 25566-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using ALKBH8 antibody (25566-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 25566-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using ALKBH8 Polyclonal antibody (25566-1-AP)

    IF Staining of U2OS using 25566-1-AP (same clone as 25566-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using ALKBH8 antibody (25566-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 25566-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

25566-1-PBS bindet in IHC, IF/ICC, Indirect ELISA ALKBH8 und zeigt Reaktivität mit human, Ratten

Getestete Reaktivität human, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen ALKBH8 fusion protein Ag22239
Vollständiger Name alkB, alkylation repair homolog 8 (E. coli)
Berechnetes Molekulargewicht 664 aa, 75 kDa
GenBank-ZugangsnummerBC015183
Gene symbol ALKBH8
Gene ID (NCBI) 91801
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ALKBH8 (Alpha-ketoglutarate-dependent dioxygenase) is unique among the ALKBH family, since it harbors a 2-oxoglutarate and Fe(II)-dependent oxygenase domain as well as an RNA recognition motif and methyl transferase domain (PMID: 27329810). ALKBH8 is a "writer" enzyme that modifies the wobble uridine of tRNA and regulates protein translation, including that of selenoproteins (PMID: 38550277). ALKBH8 is highly expressed in high-grade, superficially and deeply invasive bladder cancer. ALKBH8 knockdown induces apoptosis in bladder cancer cells (PMID: 38550277).