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ALOX15 Polyklonaler Antikörper

ALOX15 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 13286-1-AP

Synonyme

15LOX, 15 LOX2, 15 LOX 1, 15 LOX, 12-LOX

Filter:
  • Western Blot (WB) analysis of various lysates using ALOX15 Polyclonal antibody (13286-1-AP)

    WB analysis using 13286-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13286-1-AP (ALOX15 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Geprüfte Anwendungen

Erfolgreiche Detektion in WBMaus-Kolongewebe, Ratten-Kolongewebe

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

WBSee 1 publications below

Produktinformation

13286-1-AP bindet in WB, ELISA ALOX15 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen ALOX15 fusion protein Ag4040
Vollständiger Name arachidonate 15-lipoxygenase
Berechnetes Molekulargewicht 662 aa, 75 kDa
Beobachtetes Molekulargewicht70-75 kDa
GenBank-ZugangsnummerBC029032
Gene symbol ALOX15
Gene ID (NCBI) 246
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for ALOX15 antibody 13286-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

J Agric Food Chem

Combined Toxicity Assessment of Deoxynivalenol and Pb2+ on HK-2 Cells Involved in Excessive ROS-Induced Ferroptosis

Authors - Zuoyin Zhu
View Article

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Nazek (Verified Customer) (08-07-2025)

tooooo many non-specific bands seen in the western blot

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: neuroblastoma protein lysates
ALOX15 Antibody Western Blot validation (1:500 dilution) in neuroblastoma protein lysates (Cat no:13286-1-AP)