ALOX15B Polyklonaler Antikörper

ALOX15B Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(10)

Rezensionen (2)

Kat-Nr. : 13073-1-AP

Synonyme

15 lipoxygenase 2, 15-LOX-2, 15-lipoxygenase 2, 15 LOX B, 15 LOX 2

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• 

  WB analysis of A431 using 13073-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 13073-1-AP (15 Lipoxygenase 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 13073-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13073-1-AP (15 Lipoxygenase 2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HEK-293-Zellen, HeLa-Zellen, HL-60-Zellen, MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 9 publications below
• IHC: See 1 publications below

Produktinformation

13073-1-AP bindet in WB, IHC, ELISA ALOX15B und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Hausschwein, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ALOX15B fusion protein Ag3741
• Vollständiger Name: arachidonate 15-lipoxygenase, type B
• Berechnetes Molekulargewicht: 676 aa, 76 kDa
• Beobachtetes Molekulargewicht: 67-76 kDa
• GenBank-Zugangsnummer: BC035217
• Gene symbol: ALOX15B
• Gene ID (NCBI): 247
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

In humans, there are 6 functional lipoxygenases genes. Two of them encode for arachidonic acid 12-lipoxygenating (ALOX12, ALOX12B) and two for 15-lipoxygenating enzymes (ALOX15, ALOX15B). ALOX15B has been described as the most abundant 15-lipoxygenating enzyme species in human carotid atherosclerotic lesions and to be associated with cerebrovascular symptoms. (PMID:24373925). ALOX15B has 4 isoforms produced by alternative splicing with the MW of 76, 67, 69 and 73 kDa. 13073-1-AP can also recognize the mouse Alox8 protein.

Publikationen

Species	Application	Title
	WB	Nutrition; Intermittent fasting ameliorates neuronal ferroptosis and cognitive impairment in mice after traumatic brain injury; Authors - Qiuyun Yang
rat	IHC	Exp Cell Res; Lipoxin A4 and its analog attenuate high fat diet-induced atherosclerosis via Keap1/Nrf2 pathway.; Authors - Fen Xu
	WB	J Chem Neuroanat; Proanthocyanidin promotes functional recovery of spinal cord injury via inhibiting ferroptosis.; Authors - Huangao Zhou
pig	WB	Theriogenology; The role of ALOX15B in heat stress-induced apoptosis of porcine sertoli cells.; Authors - Hongyan Xue
human	WB	Redox Biol; Excessive SOX8 reprograms energy and iron metabolism to prime hepatocellular carcinoma for ferroptosis; Authors - Xue Yang
human	WB	Lipids Health Dis; Lipid peroxidation-induced ferroptosis as a therapeutic target for mitigating neuronal injury and inflammation in sepsis-associated encephalopathy: insights into the hippocampal PEBP-1/15-LOX/GPX4 pathway; Authors - Haosen Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Nazek (Verified Customer) (08-07-2025)

some non-specific bands seen in the western blot

• Applications: Western Blot
• Primary Antibody Dilution: 1:500

ALOX15B Antibody Western Blot validation (1:500 dilution) in (Cat no:13073-1-AP)

FH

Ankush (Verified Customer) (04-23-2024)

The antibody showed slightly different position. please refer to the marker lane

• Applications: Immunoprecipitation
• Primary Antibody Dilution: 1:2500
• Cell Tissue Type: monocytes

ALOX15B Antibody Immunoprecipitation validation (1:2500 dilution) in monocytes (Cat no:13073-1-AP)