5 Lipoxygenase Polyklonaler Antikörper

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• KD/KO Validated

5 Lipoxygenase Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Kat-Nr. : 10021-1-Ig

Synonyme

5 Lipoxygenase, 5 LO, 5 LOX, 5LPG, ALOX5, arachidonate 5 lipoxygenase, LOG5

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Galerie der Validierungsdaten

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  WB analysis using 10021-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 10021-1-Ig (5 Lipoxygenase antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 10021-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 10021-1-Ig (5 Lipoxygenase antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 10021-1-Ig

  HL-60 cells were subjected to SDS PAGE followed by western blot with 10021-1-Ig (5 Lipoxygenase antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of DU 145 using 10021-1-Ig

  DU 145 cells were subjected to SDS PAGE followed by western blot with 10021-1-Ig (5 Lipoxygenase antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 10021-1-Ig

  Immunofluorescent analysis of Hela cells, using ALOX5 antibody 10021-1-Ig at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: DU 145-Zellen, HL-60-Zellen, humanes Plazenta-Gewebe, K-562-Zellen, PC-3-Zellen
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 9 publications below
• IHC: See 4 publications below
• IF: See 2 publications below

Produktinformation

10021-1-Ig bindet in WB, IHC, IF, ELISA 5 Lipoxygenase und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: arachidonate 5-lipoxygenase
• Berechnetes Molekulargewicht: 674 aa, 78 kDa
• Beobachtetes Molekulargewicht: 78 kDa
• GenBank-Zugangsnummer: BC130332
• Gene symbol: ALOX5
• Gene ID (NCBI): 240
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ALOX5(arachidonate 5-lipoxygenase) is also named as LOG5, 5-LO and belongs to the lipoxygenase family. It catalyzes the first step in leukotriene biosynthesis, and plays a role in inflammatory processes. It is a 78 kDa iron-containing enzyme that translocates from the cytosol to associate with FLAP, and converts arachidonate in two steps into the unstable intermediate LTA4. It is essential for cysteinyl-leukotriene (cys-LT) production, critical mediators in asthma(PMID:12911785).

Publikationen

Species	Application	Title
mouse	WB,IHC	Front Pharmacol; Fenofibrate suppresses corneal neovascularization by regulating lipid metabolism through PPARα signaling pathway; Authors - Tong Zhou
human,mouse	WB,IHC,IF	Int Immunopharmacol; The ALOX5 inhibitor Zileuton regulates tumor-associated macrophage M2 polarization by JAK/STAT and inhibits pancreatic cancer invasion and metastasis; Authors - Wei-Min Hu
mouse	WB	Biochem Pharmacol; PPARα agonist WY-14,643 induces adipose atrophy and fails to blunt chronic ethanol-induced hepatic fat accumulation in mice lacking adipose FGFR1.; Authors - Yunhui Xu
human	WB	J Biol Chem; Inhibition of 5-lipoxygenase selectively triggers disruption of c-Myc signaling in prostate cancer cells.; Authors - Sivalokanathan Sarveswaran ; KD Validated
human	WB	PLoS One; MK591, a Second Generation Leukotriene Biosynthesis Inhibitor, Prevents Invasion and Induces Apoptosis in the Bone-Invading C4-2B Human Prostate Cancer Cells: Implications for the Treatment of Castration-Resistant, Bone-Metastatic Prostate Cancer.; Authors - Sivalokanathan Sarveswaran
rat	IHC	Exp Cell Res; Lipoxin A4 and its analog attenuate high fat diet-induced atherosclerosis via Keap1/Nrf2 pathway.; Authors - Fen Xu

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