5 Lipoxygenase Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

5 Lipoxygenase Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

CloneNo.

3B1B7

Kat-Nr. : 66326-1-Ig

Synonyme

ALOX5, 3B1B7, 5 LO, 5 LOX, 5-lipoxygenase

---

• 

  WB analysis of pig spleen using 66326-1-Ig

  pig spleen tissue were subjected to SDS PAGE followed by western blot with 66326-1-Ig (5 Lipoxygenase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of THP-1 using 66326-1-Ig

  THP-1 cells were subjected to SDS PAGE followed by western blot with 66326-1-Ig (5 Lipoxygenase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of THP-1 using 66326-1-Ig

  WB result of 5 Lipoxygenase antibody (66326-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-5 Lipoxygenase transfected THP-1 cells.

• 

  WB analysis of pig spleen using 66326-1-Ig

  pig spleen tissue were subjected to SDS PAGE followed by western blot with 66326-1-Ig (ALOX5 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung using 66326-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66326-1-Ig (ALOX5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 66326-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66326-1-Ig (ALOX5 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 66326-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66326-1-Ig (ALOX5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 66326-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66326-1-Ig (ALOX5 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 66326-1-Ig

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66326-1-Ig (ALOX5 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human spleen using 66326-1-Ig

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66326-1-Ig (ALOX5 Antibody) at dilution of 1:200 (under 40x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Hausschwein-Milzgewebe, THP-1-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungengewebe, humanes Milzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

66326-1-Ig bindet in WB, IHC, IF, ELISA 5 Lipoxygenase und zeigt Reaktivität mit Hausschwein, human

• Getestete Reaktivität: Hausschwein, human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: 5 Lipoxygenase fusion protein Ag18168
• Vollständiger Name: arachidonate 5-lipoxygenase
• Berechnetes Molekulargewicht: 674 aa, 78 kDa
• Beobachtetes Molekulargewicht: 70-78 kDa
• GenBank-Zugangsnummer: BC130332
• Gene symbol: 5 Lipoxygenase
• Gene ID (NCBI): 240
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ALOX5(arachidonate 5-lipoxygenase) is also named as LOG5, 5-LO and belongs to the lipoxygenase family. It catalyzes the first step in leukotriene biosynthesis, and plays a role in inflammatory processes. It is a 78 kDa iron-containing enzyme that translocates from the cytosol to associate with FLAP, and converts arachidonate in two steps into the unstable intermediate LTA4. It is essential for cysteinyl-leukotriene (cys-LT) production, critical mediators in asthma(PMID:12911785).

Publikationen

Species	Application	Title
human	WB	Front Pharmacol; MLL-AF4 upregulates 5-lipoxygenase expression in t(4;11) leukemia cells via the ALOX5 core promoter; Authors - Marius Hyprath
human	WB	ACS Synth Biol; Potential and Optimization of Mammalian Splice Riboswitches for the Regulation of Exon Skipping-Dependent Gene Expression and Isoform Switching within the ALOX5 Gene; Authors - Robin W Bruckhoff
human	WB,IF	Cancer Gene Ther; Knock-out of 5-lipoxygenase in overexpressing tumor cells-consequences on gene expression and cellular function; Authors - Hannah Weisser
human	WB,IHC	Life Sci Alliance; Three-dimensional growth reveals fine-tuning of 5-lipoxygenase by proliferative pathways in cancer; Authors - Tamara Göbel
human	WB	Kaohsiung J Med Sci; Magnolin alleviated DSS-induced colitis by inhibiting ALOX5-mediated ferroptosis; Authors - Ting Yao
human	IHC	Acta Diabetol; Identification of potential key lipid metabolism-related genes involved in tubular injury in diabetic kidney disease by bioinformatics analysis; Authors - Yuanshuo Fan