ALPK1 Polyklonaler Antikörper

ALPK1 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 19107-1-AP

Synonyme

8430410J10Rik, alpha kinase 1, Alpha protein kinase 1, ALPK, ALPK1, Chromosome 4 kinase, KIAA1527, LAK

---

Galerie der Validierungsdaten

• 

  WB analysis using 19107-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19107-1-AP (ALPK1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 19107-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19107-1-AP (ALPK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen, HEK-293-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 3 publications below
• IF: See 3 publications below

Produktinformation

19107-1-AP bindet in WB, IHC, IF, ELISA ALPK1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ALPK1 fusion protein Ag5320
• Vollständiger Name: alpha-kinase 1
• Berechnetes Molekulargewicht: 139 kDa
• Beobachtetes Molekulargewicht: 140 kDa
• GenBank-Zugangsnummer: BC060780
• Gene symbol: ALPK1
• Gene ID (NCBI): 80216
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Alpha-protein kinase 1, also known as alpha-kinase 1 (ALPK1), is associated with chronic kidney disease (CKD), myocardial infarction, gout and type 2 diabetes mellitus (DM). ALPK1 mutant has been reported to cause severe defects in motor co‐ordination in mice. ALPK1 enhances the expression of proinflammatory cytokines including IL-1β, IL-8 and TGF-β1 in cultured human THP1 and human embryonic kidney 293 (HEK293) cells (PMID: 31557402, PMID: 26275947). ALPK1 short isoform (108 kDa) was highly expressed in brain, spinal cord, heart, lung, spleen, thymus, small intestine, skin and testis, while detectable inskeletal muscle and kidney. ALPK1 large isoform (130 kDa) appeared in heart, lung, thymus and skin (PMID: 21208416).

Publikationen

Species	Application	Title
mouse	WB	Front Immunol; Alpk1 Sensitizes Pancreatic Beta Cells to Cytokine-Induced Apoptosis via Upregulating TNF-α Signaling Pathway.; Authors - Fei Ding
human,mouse	WB,IHC	J Dent Res; ALPK1 Aggravates TMJOA Cartilage Degradation via NF-κB and ERK1/2 Signaling.; Authors - X Liu
mouse	IHC	J Bone Miner Res; ALPK1 Accelerates the Pathogenesis of Osteoarthritis by Activating NLRP3 Signaling; Authors - Xin Liu
mouse	WB	Int Immunopharmacol; Neuroprotective effect of alpha-kinase 1 knockdown against cerebral ischemia through inhibition of the NF-κB pathway and neuroinflammation; Authors - Jing-Ying Li
mouse	IF	Mol Neurobiol; ALPK1 Expressed in IB4-Positive Neurons of Mice Trigeminal Ganglions Promotes MIA-Induced TMJ pain; Authors - Taomin Zhu
human,mouse	IHC,IF	Int Immunopharmacol; Pyroptosis of chondrocytes activated by synovial inflammation accelerates TMJ osteoarthritis cartilage degeneration via ROS/NLRP3 signaling; Authors - Xin Liu