ALY Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ALY Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen(11)

Rezensionen (1)

Kat-Nr. : 16690-1-AP

Synonyme

Ally of AML 1 and LEF 1, ALY, ALYREF, BEF, bZIP enhancing factor BEF, THO complex 4, THO complex subunit 4, Tho4, THOC4

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  WB analysis of HeLa using 16690-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16690-1-AP (ALY antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16690-1-AP

  WB result of ALY antibody (16690-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ALY transfected HeLa cells.

• 

  WB analysis of HEK-293 using 16690-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16690-1-AP (ALY Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 16690-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 16690-1-AP (ALY antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 16690-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16690-1-AP (ALY antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 16690-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16690-1-AP (ALY antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 16690-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ALY antibody (16690-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A431-Zellen, HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 10 publications below
• IF: See 1 publications below
• RIP: See 1 publications below

Produktinformation

16690-1-AP bindet in WB, IHC, IF/ICC, RIP, ELISA ALY und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ALY fusion protein Ag10044
• Vollständiger Name: THO complex 4
• Berechnetes Molekulargewicht: 257 aa, 27 kDa
• Beobachtetes Molekulargewicht: 27-30 kDa
• GenBank-Zugangsnummer: BC052302
• Gene symbol: ALY
• Gene ID (NCBI): 10189
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Cell Metab; NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis.; Authors - Mi Kyung Park
pig	WB	ACS Infect Dis; Porcine ZC3H11A Is Essential for the Proliferation of Pseudorabies Virus and Porcine Circovirus 2.; Authors - Lin Yang ; KD Validated
human	WB,IF	PLoS Pathog; TREX (transcription/export)-NP complex exerts a dual effect on regulating polymerase activity and replication of influenza A virus; Authors - Lingcai Zhao ; KD Validated
human	WB	Nature; mRNA recognition and packaging by the human transcription-export complex; Authors - Belén Pacheco-Fiallos
human	WB	Cell Death Dis; Epigenetic addition of m5C to HBV transcripts promotes viral replication and evasion of innate antiviral responses; Authors - Shuang Ding
human	RIP	Phytomedicine; Niujiao Dihuang Jiedu decoction promotes SLC7A11 m5C methylation modification against ferroptosis in acute-on-chronic liver failure; Authors - Liqiao Huang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Roy (Verified Customer) (06-15-2024)

Weak band on WB from HeLa cells extracts but specific for ALYREF/THOC4 (1/1000 dilution, Overnight incubation at 4°C)

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HeLa