AMH Rekombinanter Antikörper

AMH Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241148C8

Kat-Nr. : 84153-5-RR

Synonyme

Muellerian inhibiting factor, Anti-Muellerian hormone (AMH), Anti-Muellerian hormone, anti Mullerian hormone, Anti muellerian hormone

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  WB analysis using 84153-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84153-5-RR (AMH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 84153-5-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84153-5-RR (AMH antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 84153-5-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84153-5-RR (AMH antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 84153-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84153-5-RR against Human AMH were performed. The affinity constant is 2.16 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, Maus-Eierstockgewebe
• Erfolgreiche Detektion in IHC: human ovary cancer tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84153-5-RR bindet in WB, IHC, ELISA AMH und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: AMH fusion protein Ag5670
• Vollständiger Name: anti-Mullerian hormone
• Berechnetes Molekulargewicht: 59 kDa
• Beobachtetes Molekulargewicht: 60-65 kDa
• GenBank-Zugangsnummer: BC049194
• Gene symbol: AMH
• Gene ID (NCBI): 268
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Anti-Müllerian hormone (AMH), also called Müllerian-inhibiting substance (MIS), is a hormone that is best known for its production by fetal testes in mammals and as the inhibitor of Müllerian duct development in males. AMH is also expressed in granulosa cells of preantral and small antral follicles in the ovary. More recently, AMH has been studied for its role in ovarian folliculogenesis and as a potential marker of ovarian reserve. The deduced protein sequence of the AMH monomer contains a 25 amino acid secretion specific signal peptide and a monomeric protein of 535 amino acids that, when glycosylated at two glycosylation sites, has a molecular weight of 65-70 kDa.