AMOT Polyklonaler Antikörper

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• KD/KO Validated

AMOT Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Rezensionen (1)

Kat-Nr. : 16870-1-AP

Synonyme

KIAA1071, Angiomotin

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• 

  WB analysis of HEK-293 using 16870-1-AP

  WB result of AMOT antibody (16870-1-AP, 1:600) with si-Control and si-AMOT transfected HEK293 cells.

• 

  WB analysis of human stomach using 16870-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 16870-1-AP (AMOT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human stomach using 16870-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 16870-1-AP (AMOT antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Magengewebe, HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 1 publications below

Produktinformation

16870-1-AP bindet in WB, IF, ELISA AMOT und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: angiomotin
• Berechnetes Molekulargewicht: 118 kDa
• Beobachtetes Molekulargewicht: 130 kDa
• GenBank-Zugangsnummer: NM_001113490
• Gene symbol: AMOT
• Gene ID (NCBI): 154796
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Angiomotin belongs to the motion family of angiostatin-binding proteins. The encoded protein is expressed predominantly in endothelial cells of capillaries and larger placenta vessels. It may mediate the inhibitory effect of angiostatin on tube formation and the migration of endothelial cells toward growth factors during the formation of new blood vessels. The most abundant expression was found in the placenta and skeletal muscle. AMOT has two isoforms with MW 130 kDa (p130) and 80 kDa (p80). This antibody recognizes p130 only.

Publikationen

Species	Application	Title
human	WB	Mol Ther Nucleic Acids; Hsa_circ_0024093 accelerates VSMC proliferation via miR-4677-3p/miR-889-3p/USP9X/YAP1 axis in in vitro model of lower extremity ASO.; Authors - Xue Zhang
mouse	WB,IF	Int J Cardiol; Conditional knockout of TFPI-1 in VSMCs of mice accelerates atherosclerosis by enhancing AMOT/YAP pathway.; Authors - Jiajun Xiao
	WB	Pharmacol Res; The ketogenic diet modulates tumor-associated neutrophil polarization via the AMOT-YAP/TAZ axis to inhibit colorectal cancer progression; Authors - Xiuwei Mi

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jonasz J. (Verified Customer) (05-03-2024)

Very good and specific antibody, detecting a single band at the expected molecular weight.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T cells

AMOT Antibody Western Blot validation (1:1000 dilution) in HEK293T cells (Cat no:16870-1-AP)